Development of integrated biochemical chip using electroosmotic linear stepping actuator

本研究的最终目的是发展无相互干扰的微高性能片上液体传输机制，进而建立高度集成的微流体装置的方法或架构，这是微tas和片上实验室研究领域的重点。实验结果表明，我们研制出了高性能的低压电渗泵（EOF），其功率为15V， 240nL/min，是传统电渗泵的80倍。还研制了串级式低压高压泵，在10V、10级时达到25000Pa，提高了103-104倍。发现了一种新型的耐高压盐桥图图化光聚合凝胶。结果表明，SYTOP涂层适用于台阶区涂层。无电场泄漏EOF泵无需复杂的电压计算，可插入闭合回路和微流控装置的任意位置，有利于微流控装置的大规模集成。将其应用于本研究开发的一种无损伤细胞分选机，其优点是可以与其他微流控装置相互连接，而传统的微流控细胞分选机很难与之连接。本研究不期而遇地发现了一种新的DNA陷阱现象，有望应用于DNA芯片的预处理。EOF泵的不稳定性问题是由电极周围的水解引起的，通过改进电极结构克服了这一问题，使EOF泵的使用寿命延长了100倍。这也抑制了泡沫问题。随后，实现了完美的密封。本文还报道了一种具有通道检测功能的新架构。从这些结果来看，本研究成功地朝着上述最终目的取得了许多卓有成效的进展。

This research was conducted aiming at the final purpose of the developments of micro high-performance liquid transport mechanism on a chip without mutual interferences and then the methodology or architecture to establish highly integrated microfluidic devices, which are the key points in microTAS and Lab on a chip research fields.As results, we developed low voltage electroosmosis flow (EOF) pump with high performance of 15V, 240nL/min which was 80 times larger than conventional one. The cascade type low volt high pressure pump was also developed to achieve 25000Pa at 10V and 10 stages, which is 103-104 times higher. A new photopolymerization gel was discovered for patterning of high pressure resistant salt bridge. It was revealed that SYTOP was suitable for coating at step region. The electric field leak less EOF pump will contribute to large scale integration of microfluidic device because it can be inserted in closed flow loop and any position in microfluidic device without complex voltage calculation. It was applied to a damage free cell sorter which was also developed in this research and have an advantage that it can connect to each other or other micro fluidic devices, while traditional micro fluidic cell sorter is very difficult to connect those. A new phenomenon of DNA trap, which was coincidently discovered in this research, is expected to be applied to pretreatment of DNA chip. The instability problem of EOF pump revealed to be caused by hydrolysis around electrodes and be overcome by improving electrode structure, so that the life time was extended to 100 times long. This also suppressed the bubble problem. Subsequently, perfect sealing was achieved. A new architecture with passage detection also reported.From these results, this research successfully achieved many fruitful progresses toward the final purpose mentioned above.