Preparation of novel material as adhesive biomatrix by devising oligosaccharide units

我们的目的是通过设计寡糖单元来创造一种新型的粘附性生物基质材料。我们发展了人工糖肽和糖脂的合成方法，它们是通过化学-酶法将寡糖单元引入到多肽和甘油脂中。I.人工糖肽（人工粘蛋白）的功能设计化学-酶法合成了携带唾液酸型和唾液酸型粘蛋白聚糖的糖肽，并显示其作为糖识别的工具和探针是有用的。它们也可用作人流感病毒感染的聚合物抑制剂。流感病毒的感染显着增强通过增加分子量和唾液酸含量的glycopolymers.II.人工糖脂的功能设计人工糖脂组成的乳糖苷和磷脂的设计和制备作为模拟物的乳糖神经酰胺。乳糖基化的新糖脂易于转化为糖脂体，其乳糖残基有效地暴露在脂质体膜表面。我们的发现可能有助于改进定制的糖脂体作为生物识别现象的研究探针的合理设计。

Our purpose is to create a novel material as adhesive biomatrix by devising oligosaccharide units. We developed the synthetic methods of glycopolypeptide and glycolipid as artificial glycosonjugate mimetics, which were chemo-enzymatically introduced oligosaccharide units to polypeptide and glycerolipid.I.Functional design of artificial glycopolypeptide (artificial mucin)Glycopolypeptides carrying the glycan of asialo-type and sialo-type mucins were chemo-enzymatically synthesized and shown to be useful as tools and probes of carbohydrate recognition. They were also useful as polymeric inhibitors of infection by human influenza viruses. Influenza of infection by viruses was remarkably enhanced by increasing the molecular weight and sialic acid content of glycopolymers.II.Functional design of artificial glycolipidArtificial glycolipids composed of lactose glycoside and phospholipids were designed and prepared as mimetics of lactosyl ceramide. The lactosylated neoglycolipids were easily transformed into glycoliposomes, and their lactose residues effectively exposed on the liposomal membrane surface. Our finding may contribute to an improved rational design of tailored glycoliposomes as probes for investigation of biological recognition phenomenon.