Development of Enzyme Bleaching Agent System using Rice Hull of Biomass.

本研究考察了橙II使用RPO的脱色反应，以探讨其作为RPO -酶漂白体系的可能性。研究了在稻壳过氧化物酶（RPO）催化下，应用辣根过氧化物酶（HRP）、节真菌过氧化物酶（ARP）、乳酸过氧化物酶（LPO）和大豆过氧化物酶（SPO）对橙ⅱ的脱色效果。橙ⅱ脱色率随RPO含量的增加而增加。RPO浓度为3.4 × 10^<-7>M， 120秒内脱色90%。pH值为9.0时脱色率最高。在20℃下，RPO对橙ⅱ的脱色速度比LPO和SPO快，但脱色速度与ARP和HRP相同。四种过氧化物酶（pod）在高温下均不加速脱色。在20 ~ 80℃之间，RPO的速率常数随温度的升高而增大，60℃时是20℃的5倍，80℃时是7倍。RPO在贮藏0分钟后，脱色率显著提高，在90℃贮藏150分钟后，脱色率保持不变。在高温条件下，RPO是最稳定的POD。

This study examines de coloration reactions of Orange II using RPO in order to investigate the possibility of its application as a RPO -enzyme bleaching system.Decoloration of Orange II catalyzed by rice hull peroxidase (RPO) was examined for effects incurred through application of horseradish peroxidase (HRP), Arthromyces ramosus peroxidase (ARP), milk lactic peroxidase (LPO) and soybean peroxidase (SPO). The rate of Orange II decoloration increased with RPO content. 90% decoloration occurred within 120 seconds at RPO the concentration of 3.4 x 10^<-7>M. Decoloration rate was maximum near pH 9.0. At 20℃, RPO caused Orange II to fade more quickly than LPO or SPO, though the decoloration rate was the same as for ARP and HRP. Decoloration did not accelerate with any of the four peroxidases(PODs) at high temperature. Between 20℃ and 80℃, the rate constant of RPO greatly increased with temperature, being 5 times greater at 60℃ than at 20℃ and 7 times greater at 80℃. The decoloration rate of Orange II with RPO increased greatly subsequent to storage for 0 minutes and then at 90℃ for a period of 150 minutes, the decoloration rate of Orange II remained the same. RPO was found to be the most stable POD at high temperature.