Experimentally induced Vogt-Koyanagi-Harada disease in two Akita dogs

我们已经调查了是否Vogt-Koyanagi-Harada（VKH）样疾病可以诱导秋田犬通过免疫他们与酪氨酸酶相关蛋白1（TRP 1），并比较诱导的变化与自发发生的疾病，类似于人类VKH疾病的秋田犬。用人TRP 1的肽混合物免疫两只秋田狗。随后通过裂隙灯生物显微镜、检眼镜和荧光素血管造影（FA）观察眼睛的变化。在第一次免疫后约20个月，在第二只犬（犬2）中，在第二次免疫后3周，通过标准组织学方法研究了眼睛、皮肤和脑。两只狗在第一次免疫后3-4周发生脉络膜视网膜疾病。前房及玻璃体前部有大量炎性细胞浸润。眼底表现为地图状、多灶性渗出性视网膜脱离。从脉络膜检测到荧光素的多灶性渗漏。在组织学上，在第二次免疫后三周，在狗2的眼睛中存在渗出性视网膜脱离，并且在视网膜下腔中观察到炎性细胞。部分病变脉络膜增厚，炎性细胞浸润。在犬2的眼中观察到Dalen-Fuchs结节。免疫后3周皮肤可见色素脱失、色素弥散、毛囊和血管周围炎性细胞浸润。眼睛和皮肤的临床病程和变化与自发发生VKH病的秋田犬中观察到的非常相似。我们得出结论，在这些犬中诱导了VKH样疾病，这支持了秋田犬中自发发生的脉络膜视网膜疾病是VKH病的初步结论。

We have investigated whether a Vogt-Koyanagi-Harada (VKH)-like disease can be induced in Akita dogs by immunizing them with tyrosinase related protein 1 (TRP1), and compared the alterations induced to those of Akita dogs with a spontaneously occurring disease that resembles human VKH disease. Two Akita dogs were immunized with a peptide mixture of human TRP1. The changes in the eyes were followed by slit-lamp biomicroscopy, ophthalmoscopy, and fluorescein angiography (FA). The eyes, skin, and brains were studied by standard histological methods at about 20 months after the first immunization in one dog (dog 1), and at 3 weeks after the second immunization in the second dog (dog 2). Both dogs developed chorioretinal disease 3-4 weeks after the first immunization. Many inflammatory cells infiltrated into the anterior chamber and anterior vitreous. The fundus showed geographic, multifocal exudative retinal detachments. Multifocal leakages of fluorescein were detected from the choroid. Histologically, exudative retinal detachment was present, and inflammatory cells were seen in the subretinal space in the eyes of dog 2 taken three weeks after the second immunization. The choroid was thickened by the infiltration of inflammatory cells in some lesions. Dalen-Fuchs nodules were seen in the eye of dog 2. Depigmentation, pigment dispersion, and infiltration of many inflammatory cells around hair follicles and vessels were seen in the skin taken three weeks postimmunization. The clinical course and changes in the eyes and skin were very similar to those seen in the Akita dogs with spontaneously occurring VKH disease. We concluded that a VKH-like disease had been induced in these dogs, and this supports the tentative conclusion that the spontaneously occurring chorioretinal disease in Akita dogs is VKH disease.