Studies on regenerative medicine by using bone marrow stem cell in intractable hepatic diseases

1)确定了犬c-蛋氨酸的完整编码。C-Met基因与其他动物的同源性很高。C-Met是犬实验性肝部分切除后肝再生的重要因素。2)犬c-Met在部分血液系统恶性肿瘤中起一定作用，有望成为治疗肿瘤的靶点。3)犬骨髓细胞经HGF或人胎盘提取液培养后可分化为肝细胞样细胞。在BM细胞的分化过程中，HGF被认为是一个重要的因素，也被认为是人胎盘提取液中支持HGF功能的其他因素(正在申请专利)。4)对人胎盘提取液中未知因素的进一步分析将有助于再生医学的临床试验。这项研究的结果不仅对兽医科学，而且对医学科学都是重要的信息。

Next results were obtained by this studies.1) Canine c-Met complete code was determined. c-Met cDNA was showed high homology among that of other animals. c-Met was indicated to be an important factor for canine liver regeneration in experimental partial hepato-ectomy.2) Canine c-Met was suggested to play a role in a part of hematological malignancies, and expected to be a target for the treatment of tumors.3) Canine bone marrow (BM) cells were able to differentiate to hepatocyte-like cells by culture with HGF or human placental extract. In BM cell's differentiating process, HGF was identified as an important factor, and also some other factors in human placental extract were suggested to support HGF functions, (in applying for a patent)4) Further analysis of unknown factors contained in human placental extract would contribute clinical trial of regenerative medicine. The results obtained from this study will be important information not only for veterinary science but also for medical science.