Clarification of the relationship between the cytokine gene polymorphism and the rejection/tolerance after liver transplantation

在一些研究中，细胞因子基因多态性与细胞因子的产生和移植结果有关。但只有少数研究调查了患者的细胞因子谱断奶免疫抑制。在本研究中，我们分析了活体肝移植受者在脱离免疫抑制后il - 10、il - 2、il - 6、ifn - γ和tnf - α基因多态性的相关性。材料与方法：成功解除免疫抑制的肝移植受者（n=19）。采用序列特异性引物PCR（PCR- ssp）方法，测定了肝移植受者的细胞因子基因多态性：IL10-592、IL10-819、IL10-1082、IL2-330、IL6-174、IFNγ+874和TNFα-308。结果研究表明，低IFNγ+874基因型（G/A基因型）多态性与需要长时间免疫抑制有关。结论需要维持免疫抑制的患者更有可能具有IFNγ（G/ a基因型）多态性，这一结果与该细胞因子的促炎特性一致，但先前的报道显示与肾和肝移植的急性移植排斥反应无关。所以我们需要分析更多的样本和其他细胞因子。

BackgroundCytokine gene polymorphisms have been associated with cytokine production and transplant outcome in some studies. But only few studies have investigated the cytokine profile of patients weaning off immunosuppression. In this study we analyzed the association of gene polymorphisms in IL10,IL2,IL6,IFNγ and TNFα after weaning off immunosuppression in living donor liver transplant recipienta.Materials & methods:Liver transplant recipients who were successfully withdrawn from immunosuppression(n=19). A control group of liver transplant recipients who required maintenance of immunosuppression(n=30)Using the sequence specific primer PCR(PCR-SSP) method, the following cytokine gene polymorphisms were determined : IL10-592,IL10-819,IL10-1082,IL2-330,IL6-174,IFNγ+874 and TNFα-308.ResultOur study showed that Polymorphisms of low IFNγ+874 profile(G/A genotype) was associated with requiring prolonged immunsuppression.ConclusionPatients that require maintained immunosuppression are more likely to have a polymorphism of IFNγ(G/A genotype)This result is consistent with the proinflammatory properties of this cytokine, but previous reports showed no association with acute graft rejection in kidney and liver transplantation. So we need to analyse more sample and other cytokines.