Angiogenesis and Tumor Proliferation/Metastasis of Human Colorectal Cancer Cell Line SW620 Transfected with Endocrine Glands-Derived-Vascular Endothelial Growth Factor, As a New Angiogenic Factor

内分泌腺源性静脉内皮细胞生长因子(EG-VEGF)是新近克隆的一种选择性作用于内分泌腺细胞内皮细胞的新型血管生成因子。所有结直肠癌细胞系均表达EG-VEGF。(另一方面，在正常大肠粘膜中未检测到EG-VEGFmRNA的表达。)EG-血管内皮生长因子转染组明显高于对照组。此外，EG-VEGF转染组的肝转移率也高于对照组。在本研究中，EG-VEGF可能导致结直肠癌血管生成、促进细胞增殖和肝转移。用硫代反义EG-VEGF寡核苷酸处理过表达EG-VEGF基因的结直肠癌细胞系，血管生成和肿瘤生长均受到抑制。血管生成因子EG-VEGF表明，它是一种肿瘤特异性的、可能是组织特异性的大肠血管生成因子，并提示它可以成为一种新的抗血管生成治疗的靶点。

Endocrine glands-derived-venous endothelial growth factor (EG-VEGF) was recently cloned as a new angiogenic factor that selectively acts on the endothelium of endocrine gland cells. The expression of EG-VEGF was comfirmed in all colorectal cancer cell lines. (On the other hand, the expression of EG-VEGF mRNA was not detected in colorectal normal mucosae.)Tumor proliferation(cecum, s.c.) was significantly higher in the EG-VEGF transfectants than in the control cells. Also, Liver metastatic ratio was higher in the EG-VEGF transfectants than in the control cells. In this study, EG-VEGF may lead to angiogenesis, promoting cell proliferation, and liver metastasis in colorectal cancers. And When the EG-VEGF gene-overexpressing colorectal cancer cell line had been treated with phosphorothioate antisense EG-VEGF oligonucleotides, angiogenesis and tumor growth were inhibited. Angiogenesis factor EG-VEGF indicates that it is a cancer-specific and possibly tissue-specific angiogenesis factor in the large intestine, and suggests that it can be targeted by a novel anti angiogenesis therapy.