A role of osteopontin in ulcerative colitis

溃疡性结肠炎（UC）是一种病因不明的多因素疾病。很少有研究在UC的结肠组织样本中应用全基因组基因表达分析。我们使用高密度寡核苷酸阵列系统（Affyssin GeneChip）对UC和非炎症对照标本中的基因表达进行了分析。System，Santa Clara，CA）。另外，使用针对在UC的结肠组织中最显著表达的基因产物的抗体，在溃疡性结肠炎（UC）、克罗恩病（CD）和憩室炎（Div）患者的结肠组织中进行免疫组织化学（IHC）研究。研究方法：为了比较UC和对照样品之间基因表达水平的差异，对10个结肠组织样品（7个UC和3个正常对照样品）进行高密度寡核苷酸阵列分析。另外，对UC组、CD（克罗恩病）组、Div（憩室炎）组三组中显著表达的基因产物进行了IHC染色研究 ...更多信息 ）组。结果：1）UC组织中有25个基因的mRNA表达量是正常组织的3.0 ~ 23.4倍。在这25个基因中，只有两个基因的变异超过10倍，其中骨桥蛋白（OPN）表达最强。2)关于肠上皮细胞中通过IHC的OPN表达，在三组之间没有观察到差异。而UC组黏膜下层OPN表达的两种大细胞（椭圆形和梭形）的比例为61.2±14.4%（mean±SD），显著高于CD组（14.9±7.0%）（p<0.05）和Div组（11.2±6.1%）（p<0.05）。此外，UC组浆膜下的比例（50.1±15.0%）（平均值±SD）显著高于CD组（16.9±6.2%）和Div组（12.6±5.7%）。基于连续切片研究，椭圆形细胞针对抗CD 68染色，而梭形细胞针对抗波形蛋白染色。结论：我们目前的研究表明，骨桥蛋白可能通过增加免疫活性在UC的发病机制中发挥重要作用。少

Ulcerative colitis(UC) is a multifactorial disorder of unknown etiology. Few studies have applied genome-wide gene expression analysis in colon tissue samples of UC. We performed the analysis of gene expression in UC and noninflamed control specimens using high-density oligonucleotide array system (Affymetrix GeneChip^<【○!R】> System, Santa Clara, CA). In addition, using the antibody against the gene product that was expressed most remarkably in the colon tissue of UC, immunohistochemistry(IHC) study was performed in colon tissues of ulcerative colitis(UC), Crohn's disease(CD) and diverticulitis(Div) patients. Methods : To compare differences in the level of gene expression between UC and control samples, 10 colonic tissue samples (7 UC and 3 normal control samples) were subjected to high-density oligonucleotide array analysis. In addition, IHC staining study for the remarkable expressed gene product was performed in three groups : UC group, CD(Crohn's disease) group, Div(diverticulitis … More ) group. Results : 1)Twenty five genes had a 3.0〜23.4-fold higher mRNA expression in UC samples compared with normal samples. Among the 25 genes, only two genes varied by more than 10-fold and a gene with the strongest expression was osteopontin(OPN). 2)Regarding OPN expression by IHC in intestinal epithelial cells, no difference was noted among the three groups. However, in the submucosa of the UC group, the ratio of two types of large cells (oval and spindle shape) expressing OPN was 61.2±14.4% (mean±SD), which was a significantly higher ratio than the CD group (14.9±7.0%) (p<0.05) and the Div group (11.2±6.1%) (p<0.05). In addition, the ratio of the UC group in the subserosa (50.1±15.0% )(mean±SD) was significantly higher than the CD group (16.9±6.2%) and Div group (12.6±5.7%). Based on the serial section study, oval shape cells were stained for anti-CD 68,while spindle shape cells were stained for anti-vimentin. Conclusion : Our present study indicates the possibility that osteopontin plays an important role in one of the pathogenesis of UC via increased immune activity. Less