Endobronchial targeting therapy using Perfluorocabon-mediated drug delivery system to lung neoplasm

与其他肿瘤患者相比，肺癌患者的生存率仍然不令人满意。这些患者中的大多数在肺癌治疗期间死于呼吸功能不全，这些肺癌的基础是肺气肿或间质性肺炎。除肺癌组织外，理想的肺癌治疗不应引起任何肺损伤。全氟卡宾是一种良好的低表面张力氧载体，可以选择性地作为肺癌的药物缓释剂。作为初步研究，我们阐明了全氟卡宾介导的类固醇缓释系统对盐酸盐滴注和内毒素注射所致急性肺损伤的疗效。在第二步中，我们通过转移性Sato肺癌大鼠模型评价了全氟碳介导的药物传递系统在肺癌治疗中的作用。将佐藤肺癌静脉注射到Donryu大鼠模型后，用荧光活体显微镜观察肺癌的微循环。静脉注射内毒素后，在转移性肺癌周围区域发生微循环障碍，包括活化的中性粒细胞的隔离。与那些未经任何治疗的微循环障碍相比，全氟碳素介导的递送系统可减轻这些微循环障碍。综上所述，本研究结果表明，全氟化碳介导的药物传递系统可用于肺癌的临床治疗。

Survival rate of patients with lung cancer remains unsatisfied compared to those of patients with other neoplasms. Most of these patients died of respiratory insufficiency during lung cancer treatment based on such underlying lung diseases as pulmonary emphysema or interstitial pneumonia. Ideal lung cancer treatment should not induce any lung injury except lung cancer tissue. Perfluorocabon is a good agent as oxygen delivery with low surface tension and can be utilized as drug delivery agents selectively to lung cancer tisuue.We clarified efficacy of Perfluorocabon-mediated steroid delivery system to acute lung injury caused by hydrochloride instillation and endotoxin injection as a pilot study. In the second step, we evaluated utility of Perfluorocabon-mediated drug delivery system to lung neoplasm by means of metastatic Sato-lung cancer rat models. Microcirculation of lung cancer implanted after intravenously injection of Sato-lung cancer to Donryu-rat models was visualized via a fluorescent vital microscope. Microcirculatory disorders including sequestration of activated neutrophils occurred in the regions around metastatic lung cancer after intravenously injection of endotoxin. Perfluorocabon-mediated delivery system attenuated these microcirculatory disorders compared to those microcirculatory disorders without any treatments. In conclusion, the result suggested that Perfluorocabon-mediated drug delivery system can be clinically utilized in the lung cancer treatment.