Effects of aging on the actions of general anesthetics in hippocampal slice

我们比较了4周龄（幼龄组）和18周龄（老龄组）大鼠海马CA1区全麻作用的年龄相关性改变。雄性Wistar大鼠海马横向切片（400 μm）。在Schaffer侧支纤维上放置两组双极电极，以引发CA1区域的群体峰值（PS） [Sch]，并在海马体肺泡上激活抑制性中间神经元以进行gaba能调节[Alv]。挥发性麻醉剂（异氟醚、七氟醚）降低PS[Sch]，而静脉麻醉剂（硫喷妥钠、异丙酚）对PS的影响最小[Sch]。挥发性麻醉药的抑制作用程度在年轻组和老年组之间相似。另一方面，挥发性和静脉麻醉药均可降低PS[Sch+Alv]。年轻组的动作明显大于年长组。我们证明了全麻的年龄依赖性改变。在体外大鼠中枢神经系统制剂中，对抑制性突触传递有作用，而对兴奋性突触传递无作用。

We compared age-related modifications of the effects of general anesthetics in area CA1 of hippocampal slices at 4 weeks (younger group) and 18 weeks (elder group) of age.Method. Transverse hippocampal slices (400 μm) were prepared from male Wistar rats. Two sets of bipolar electrodes were placed on Schaffer collateral fibers to elicit population spikes (PS) in area CA1 [Sch], and on alveus hippocampi to activate the inhibitory interneurons for GABAergic modulation [Alv].Results. Volatile anesthetics (isoflurane, sevoflurane) decreased PS[Sch], while intravenous anesthetics (thiopental, propofol) had a minimal effects on PS[Sch]. The extents of inhibitory effects by volatile anesthetics were similar between younger and elder groups. On the other hand, both volatile and intravenous anesthetics reduced PS[Sch+Alv]. The actions were grater on younger group than on elder group.Conclusion. We demonstrated the age-dependent modification of general anesthetic. actions on the inhibitory synaptic transmission, but not on the excitatory synaptic transmission, in rat CNS preparations in vitro.