Monitoring of CD15s Ag in Clinical Renal Tx and xenogereic model.

跨血屏障肾移植是研究不同碳水化合物抗原的体液反应的独特模型。然而，在这样的肾移植中，B细胞的特性以及B细胞产生的抗体都不太明确。在本研究中，我们通过流式细胞术分析研究了B细胞亚群，即CD5(+) B-1和CD5(-) B-2亚群，并通过ELISA检测了它们的抗体亚类，这些患者接受了跨血屏障肾移植。研究对象包括5例功能良好的受体（1组）和5例abo血型不相容肾移植术后伴有抗体滴度升高的移植物丧失受体（2组）。b细胞群分析显示，两组患者的CD5(+) B-1细胞在移植后不久短暂增加，而CD5(-) B-2细胞仅在移植后1个月显著增加。抗体亚类分析显示，IgG2和IgM在1组中轻度升高，而IgG2、IgM和IgG1在2组中明显升高。

Renal transplantation across the blood barrier is a unique model for investigating the humoral response to different carbohydrate antigens. However, in such a renal transplantation, the characteristics of B cells as well as of the antibodies produced by B cells are less well defined.In the present study we investigated B cell subsets, i.e., the CD5(+) B-1 and CD5(-) B-2 subsets, by flow cytometric analysis, and their subclasses of antibody, by ELISA, in patients who had undergone renal transplantation across the blood barrier. The subjects consisted of five recipients with good function (group 1) and five recipients with graft loss (group 2) accompanied by antibody-titer elevation after ABO-incompatible renal transplantation.The B-cell population analysis revealed that CD5(+) B-1 cells temporarily increased in all patients in both groups soon after transplantation, and that CD5(-) B-2 cells significantly increased one month after transplantation only in group 2. The antibody subclasses analysis showed mild elevation of IgG2 and IgM in group 1 as opposed to remarked elevation of IgG2,IgM and IgG1 in group 2.