Estimation of cancer susceptibility and risk of head and neck carcinoma by gene polymorphism and expression.

代谢致癌物质、修复DNA损伤和诱导细胞凋亡的能力的遗传差异可能导致整个人群中癌症风险的变化。DNA修复基因的功能可能在烟草相关癌的发生中起重要作用.我们推测XPD/ERCC 2外显子23和XRCC 1密码子26304的遗传多态性可能与SCCHN的遗传易感性有关。采用PCR-RFLP方法检测这些多态性。XPD/ERCC 2 exson 23多态性检测结果如下：对照组：野生型27例（93.1%），异质型2例（6.9%），突变型0例（0%）;喉癌患者：野生型54例（91.5%），异质型5例（8.5%），突变型0例（0%）。XRCC 1密码子26304多态性结果如下，对照：野生型25例（86.2%），杂合型4例（13.8%），突变型0例（0%），患者：野生型52例（88.1%），杂合型6例（10.2%），突变型1例（1.7%）。XPD/ERCC 2、XRCC 1基因多态性与喉癌易感性无显著相关性。我们的结论是，这些多态性不是Tablets相关癌症易感性的指标。

Genetic differences in the ability to metabolize carcinogens, repair DNA damage and induce apoptosis may contribute to the variation in cancer risk in population at large. The capacity of DNA repair genes may a play important role in carcinogenesis with Tabacco- related cancer. We hypothesize that inherited polymorphisms of XPD/ERCC2 exson 23 and XRCC1 codon 26304 may contribute to genetic susceptibility to SCCHN. These polymorphisms were investigated with PCR-RFLP method. The result of XPD/ERCC2 exson 23 polymorphisms are below, control : wild home type 27 cases (93.1%), hetero type 2 cases (6.9%), mutant type 0 case (0%), patient (laryngeal carcinoma ; SCC) : wild home type 54 cases (91.5%), hetero type 5 cases (8.5%), mutant type 0 case (0%). The result of XRCC1 codon 26304 polymorphisms are below, control : wild home type 25 cases (86.2%), hetero type 4 cases (13.8%), mutant type 0 case (0%), patient : wild home type 52 cases (88.1%), hetero type 6 cases (10.2%), mutant type 1 case (1.7%). There were no signicicant between XPD/ERCC2, XRCC1 polymorphisms and susceptibility to laryngeal carcinoma. We concluded that these polymorphisms are not indicator of susceptibility to Tabacco- related cancer.