Basic and Clinical Studies about the Spectroscopical Diagnostic Technology of Ortho-Oral Diseases

在光谱诊断技术中，使用了荧光光谱诊断和在可见光波长范围内对活检组织进行染色组织的诊断以及病理检测。在这项光谱诊断技术的投影研究中，目的是将红外区域冻结切片中我们无法检测到的成分成像作为一种新的诊断技术。[1]龋病的拉曼显微光谱分析：(1)在羟基磷灰石的P=O对称拉伸成分P=O峰处测绘龋齿表面牙釉质和牙本质的边界。结果表明，牙釉质中钙羟基磷灰石的含量是牙本质中的2倍。(2)牙釉质和牙本质区龋区羟基磷灰石含量均下降。(3)观察到相同的边界地区…进一步通过扫描电子显微镜(SEM)和元素分布的X射线(EDX)探测器的磷和钙原子，以获得图像对比牙釉质和牙本质。[2]唾液痂的拉曼光谱测量：(1)在拉曼显微光谱分析的结果中，在钙化部位观察到磷酸(P=O)的吸收峰，峰的图谱显示为一环，环的中心区域从钙化处脱落。(2)标本干燥过程中，用傅立叶变换红外光谱仪ATR法测定患者唾液中的唾液含量。利用红外光谱分析软件对唾液蛋白的二级构象组成进行了分析。较好的片材组成比随着干燥的增加而增加，这与正常唾液情况下的减少不同。较少

Fluorescence spectroscopic diagnosis and a diagnosis of staining tissue sampled with a biopsy in the region of visible wavelength were used well as a pathological detection in the spectroscopic diagnosis technology. In this projected studies of spectroscopic diagnosis technique, it is the aim to take imaging of the components in the frozen section in the field of infrared region which we can not detect as a new diagnosis technique. In the results, phenomena and knowledge were observed during two years as follows :[1]Raman Microspectroscopic Measurements of Caries : (i) The border area of enamel and dentin on a caries tooth were mapping at the peak of P=O symmetrical stretching component of PO_4^<2-> of calcium hydroxyapatites. It resulted that the content of calcium hydroxyapatites in enamel area was higher two times than that in dentin. (ii) The content of calcium hydroxyapatites was dropped out in caries region on both enamel and dentin areas. (iii) The same border area was observed … More by a scanning electron microscopy (SEM) and an element distribution of X-ray (EDX) detector of P and Ca atoms to get an image contrast between enamel and dentin. Raman mapping image got an image contrast in the calcium hydroxyapatites distribution in enamel area instead of the EDX images of P and Ca atoms which the distribution contrast were not clear.[2]Raman Microspectroscopic Measurements of Salivary Caliculus : (i) In the results of measurement of salivary caliculus by a Raman microspectroscope, it was observed the phosphoric acid (P=O) absorption peak at the calcified area, and the mapping of the peak showed a ring and the center area of ring was dropped out of the calcification. (ii) The saliva liquid of the patient for salivary caliculus was measured by an ATR method of FT-IR spectrophotometer during drying the sample. The second conformation components of the saliva liquid protein were analyzed the FT-IR spectrum by a software. The Better sheet component ratio against the other ones increased with drying, which were different of decrease in the case of normal saliva liquid. Less