The cross talking of the gene and regeneration in regeneration of periodontal tissue - main role of CBFA-1 -

核心结合因子A-1（CBFA-1）缺乏可导致锁骨颅骨不连，并在成骨细胞前体细胞向成骨细胞分化中起重要作用。CBFA-1在许多组织中表达，并控制细胞的分化和生长。在戊巴比妥钠麻醉下，40 g Wistar大鼠经左心室灌流4%多聚甲醛溶液（0.025M磷酸盐缓冲液，pH7.4），用原位杂交和免疫组织化学方法研究了BMP和CBFA-1 mRNA的相互作用。胚胎用0.1M EDTA脱矿（pH 7.4），并在乙醇组织中脱水后，这些动物实验是由动物实验伦理委员会批准的，并在日本大学医学院动物实验指导方针的控制下适当地进行。在口腔颌面部的发育过程中，骨和牙齿形成区域的前成骨细胞中出现BMP的表达。CBFA-1 mRNA的表达比BMP mRNA的表达稍晚。CBFA-1 mRNA在成骨细胞和骨细胞中表达。在成牙骨质细胞中GDF呈强阳性表达，在牙根形成过程中，BMP mRNA在上皮根鞘（ERSH）细胞中表达。CBFA-1 mRNA在ERSH面的腭突细胞和前牙本质细胞中表达，BMP的表达刺激CBFA-1 mRNA的转录水平。因此，ERSH中BMP的表达可诱导ERSH对面的腭突细胞和前牙本质细胞表达CBFA-1。BMP和CBF-1的交互作用调节形成根形成和骨的细胞的分化。

Deficiency of Core binding factor A-1(CBFA-1) causes cleidocranial disostosis and play important role differentiation of the osteoblasts precursor cells to the osteoblast. CBFA-1 is expressed in many tissues and controls differentiation and growth of cells. Expression of CBFA-1 mRNA is regulated by the expression of BMP.In the present study, cross talking of BMP and CBFA-1 mRNA were studied by in situ hybridization and immunohistochemistry.Under sodium pentobarbital anesthesia, Wistar rats (40 g) were perfused via the left ventricles with 4% paraformaldehyde solution diluted in 0.025 M phosphatebuffer (pH 7.4). The embryos were demineralized with 0.1M EDTA (pH 7.4), and after dehydration in ethanol tissue, they were embedded in paraffin and then cut longitudinally into 5 μm thick slices along the long axis of incisors.These animal experiments were approved by the Institutional Ethical Committee for Animal Experiments and properly carried out under the control of the Guidelines for Animal Experimentation in Nihon University School of Dentistry.During the development of oralmaxillo-facial region, expression of BMP was appeared in preosteoblasts of the region of the bone and tooth formation. Expression of CBFA-1 mRNA was detected slightly later stage than those of BMP mRNA. CBFA-1 mRNA was expressed in the osteoblasts and osteocytes. GDF was strongly expressed in the cementoblasts.During root formation, BMP mRNA was expressed in the cells of epithelial root sheath of Hertwig (ERSH). On the other hand, CBFA-1 mRNA was expressed in the dental palilla cells and preodontoblasts facing the ERSH.Expression of BMP stimulates transcription level of CBFA-1 mRNA. Therefore, the expression of BMP from the ERSH induce the expression of CBFA-1 of n the dental palilla cells and preodontoblasts facing the ERSH. The cross talking of BMP and CBF-1 regulate the differentiation of the cells forming root formation and bone.