The Basic research on the probiotics for the prevention of the periodontal disease and halitosis.

通过体外实验，发现与乳杆菌或粪肠球菌共培养对牙龈卟啉单胞菌的生长有抑制作用。然而，这可能是由于这些细菌的培养伴随着pH值的降低。在口腔中，很难认为这些细菌控制了一些口腔液的pH值。益生菌的作用应从另一个方面考虑。因此，我们将变形链球菌MT8148、OMZ175或远缘链球菌6715、33478和卡氏乳链球菌(S.mitis)6249、粪肠球菌BIO 3B按不同比例混合培养。通过共培养，变形链球菌和远缘链霉菌产生的水不溶性葡聚糖按比例的剂量效应方式受到抑制。然而，共培养并没有抑制变形链球菌和远缘链霉菌的生长。此外，与粪肠球菌共培养对变形链球菌和远缘链霉菌的集落形成也有抑制作用。根据这些结果，我们认为益生菌对牙周病或口臭的作用可能是通过抑制生物被膜的产生等间接作用而不是直接抑制牙周细菌的生长来实现的。

By the in-vitro investigation, the growth of the Porphyromonas gingivalis was suppressed by the co-cultivation with Lactobacillus or Enterococcus faecali (E.faecalis). However, this may be due to the lowering pH accompanied by the cultivation of these bacteria. In the oral cavity, it is hard to consider these bacteria control the pH of the some of the oral fluid. The effect of probiotics should be considerd by the another aspects. Thus, we co-cultivate Streptococcus mutans (S.mutans) MT8148,OMZ175 or Streptococcus sobrinus (S.sobrinus) 6715, 33478 and L.casei IAM12473 Streptococcus mitis (S.mitis) 6249, Enterococcus faecalis (E.faecalis) BIO 3B by several proportions of these bacteria. By the co-cultivation, the production of the water insoluble glucans produced by the S.mutans and S.sobrinus were suppressed according to the dose response manner of the proportion. However, the growth of the S.mutans and S.sobrinus were not suppressed by the co-cultivation. In addition, the co-cultivation with E.faecalis the colony forming of the S.mutans and S.sobrinus were inhibited. According to these results, we concluded that the effect of the probiotics for the periodontal disease or halitosis may be derived form the indirect effect such as inhibition of the producing the biofilms rather than direct effect to suppress the growth of the periodontal bacteria.