Dynamic status and senescence of hepatic stem cells in injured rat liver

间变性甲状腺癌(ATC)的预后更差，而且他们没有通过手术治愈，从疾病中存活的机会非常低。TB开发了治疗ATC的新策略，我们检测了ATC细胞中Chk1抑制物(UCN-01)的表达，并用Western blotting检测了ART、ATM和Chk1在ATC细胞中的表达和/或磷酸化状态。ATC细胞经UCN-01处理后出现明显的抑制和凋亡，并呈剂量和时间依赖关系。此外，我们还证实了ART、ATM和Chk1磷酸化的下调。与乳头状甲状腺癌细胞系相比，MIR-138在ATC细胞系中的表达显著下调。我们的临床前试验表明，UCN-01对ATC患者的治疗有一定的作用，miR-138的表达可能调节Chk1的磷酸化状态。

Anaplastic thyroid cancer (ATC) has an even worse prognosis, and they have not cured by the surgery with a very low chance of surviving from the disease. Tb develop a new strategy for the treatment of ATC, we examine Chk1 inhibitor (UCN-01) in ATC cell lines.We determined expression and/or phosphorylation status of ART, ATM, and Chk1 in ATC cell lines using Western blotting. Proliferation activities were measured by ATP assays.Marked repression and apoptosis was observed in ATC cells after the treatment with UCN-01, in dose- and time dependent manner. Furthermore, we confirmed down-regulation of ART, ATM, and Chk1 phosphorylation. MiR-138 was significantly down-regulated in ATC cell lines in comparison with papillary thyroid cancer cell lines. Its loss of miR-138 was closely associated with Chk1 status in ATC cell lines.Our preclinical trials suggested that UCN-01 was useful for the management of ATC patients, and the miR-138 expression might regulate the Chk1 phosphorylation status.