Bovine Leukemia Virus: A novel Zoonosis?

牛白血病病毒（BLV）与人T细胞白血病病毒（HTLV）密切相关，大量牛被感染，因此，明确人是否也能感染BLV具有重要意义。(1)BLV前病毒DNA（pDNA）的检测BLV pDNA采用PCR方法从外周血单个核细胞（PBMC）DNA中扩增，Southern印迹法（SB）鉴定，实时荧光定量PCR检测。虽然在少数样品中几乎检测不到BLV pDNA，但验证性实验的结果不一致。考虑到前病毒载量可能在阈值水平附近，我们通过用EB病毒（EBV）转化外周血B细胞来扩增被认为是BLV靶向细胞的外周血B细胞。用大量的健康人EB病毒转化的B细胞DNA样品，对BLV的tax、env和pol基因进行了扩增和序列测定。 ...更多信息 鉴定的BLV pDNA与美国和澳大利亚的接近，与日本奶牛中发现的几乎相同。然而，迄今为止，BLV pDNA的更多延伸区域的扩增尚未成功，部分原因是PCR困难和低拷贝数。(2)抗BLV抗体的检测从持续BLV感染细胞的裂解物或从这些细胞的无细胞培养上清液制备BLV蛋白。此外，从大肠杆菌中纯化了与GST融合的BLV Tax或Env蛋白。大肠杆菌培养物。将这些BLV蛋白用作蛋白质印迹（WB）的抗原以检测人血清样品中的抗BLV抗体。根据HTLV WB的诊断标准，11名健康志愿者中有1例（9%）阳性，另1例（9%）不明确，32例乳腺癌患者中有1例（3%）阳性，3例（9%）不明确。然而，BLV pDNA的存在并没有被证实在BLV血清阳性individuals.Discussion：从最近报道的研究在美国，有很多BLV血清阳性的个人和抗BLV抗体的存在并不能预测BLV pDNA的存在在他们的外周血B细胞不同。因此，BLV感染可能是罕见的，如果有的话，在人类。少

Bovine leukemia virus (BLV) is closely related to human T-cell leukemia virus (HTLV), and considerable number of cattle have been shown to be infected with this virus; therefore, it is critical to clarify if human being can also be infected with this virus.(1) Detection of BLV provirus DNA (pDNA)BLV pDNA was amplified from peripheral blood mononuclear cells (PBMC) DNA with PCR and confirmed with Southern blotting (SB), or quantitated with real-time PCR. Although barely detectable BLV pDNA was found in a few samples, results of confirmatory experiments were inconsistent. Considering that proviral load might be around threshold level, we expanded peripheral blood B cells that are supposed to be BLV-targeting cells by transforming them with Epstein-Barr virus (EBV). Using large amount of DNA samples of EBV-transformed B cells derived from a healthy volunteer, we could amplified and sequenced several BLV genes such as tax, env and pol. Phylogenetic analysis of BLV env gene demonstrated the … More identified BLV pDNA was close to those in the USA and Australia and almost identical to those found in cows in Japan. However, amplification of more extended regions of BLV pDNA has been unsuccessful so far, partly because of difficulty in PCR and low copy number.(2) Detection of anti-BLV antibodyBLV proteins were prepared from lysates of persistently BLV-infected cells or from cell-free culture supernatants of those cells. In addition, recombinant BLV Tax or Env protein was purified as fusion proteins with GST from E. coli cultures. Those BLV proteins were used as antigens for Western blotting (WB) to detect anti-BLV antibodies in human serum samples. Based on the diagnostic criteria for HTLV WB, one (9%) was positive and another (9%) was ambiguous among 11 healthy volunteers, and one (3%) was positive and three (9%) were ambiguous among 32 patients with breast cancer. However, the presence of BLV pDNA was not confirmed in BLV-seropositive individuals.Discussion: Different from a recently reported study in the USA, there were not many BLV-seropositive individuals and the presence of anti-BLV antibody did not predict the presence of BLV pDNA in their peripheral blood B cells. Thus, BLV infection is probably rare, if any, in humans. Less