Modeling actin-microtubule crosstalk during centrosome positioning in cell migration and division

中心体的正确定位在有丝分裂、细胞内货物运输、迁移和免疫细胞激活和杀伤过程中的极化过程中至关重要。越来越清楚的是，微管和肌动蛋白细胞骨架在这一核心细胞过程中共同工作，它们的功能动态特性经常紧密交织在一起。在这个项目中，我们的目标是了解细胞几何结构、微管、肌动球蛋白网络和这些组件之间的串扰在定位间期细胞中中心体的作用。我们计划开发一个计算模型来研究动态变化的细胞形状和肌动蛋白皮质产生的力在二维和三维静态和迁移细胞中对中心体定位的影响。反之亦然，我们知道中心体的位置与迁移细胞的运动方向密切相关，我们打算用我们的计算模型来分析这一点。我们的最终目标是确定肌动蛋白-微管串扰机制，使阿米巴迁移细胞能够在复杂的三维环境中导航，如细胞外基质。

The correct positioning of the centrosome is crucial during mitosis, intracellular cargo transport and polarization in migration and immune cell activation and killing. It is becoming increasingly clear that the microtubule and actin cytoskeleton work together in this core cellular processes and that their functional dynamic properties are often intimately intertwined. In this project, we aim to understand the role of cellular geometry, microtubule, actomyosin network, and crosstalk among these components in positioning the centrosome in the interphase cell. We plan to develop a computational model to study the influence of a dynamically changing cell shape and actin cortex generated forces on the centrosome positioning centrosome in static and migrating cells in two and three dimensions. Vice versa it is known that centrosome position strongly correlates with the direction of movement in migrating cells, which we aim to analyze with our computational model. Our ultimate goal is to identify the actin-microtubule crosstalk mechanisms that enables amoeboid migrating cells to navigate through a complex three-dimensional environment like extracellular matrices.