Use of urinary bilirubin oxidative metabolites as a psychological stress-marker.

一些作者认为，心理应激诱导活性氧（ROS）的产生。一些研究支持胆红素在体内发挥抗氧化作用。然而，尚不清楚心理应激诱导的ROS是否引起体内胆红素氧化。我们调查了暴露于心理压力的受试者尿液中胆红素氧化代谢产物生物吡咯的浓度是否增加。在一家制药公司工作的60名健康男性志愿者被分为不参加会议的第一组，参加会议但不发表演讲的第二组，以及参加会议并在公司高管在场的情况下发表演讲的第三组。在第三组的受试者在会议上发表演讲后，对主观压力进行评分（自评评分）。第二天收集尿液。通过酶联免疫吸附测定法测量的生物比林浓度被标准化为尿肌酐浓度。第III组中生物吡林的浓度明显高于第I组和第II组（分别为p<0.01和p<0.05）。此外，在第III组中，生物比林浓度与自评应激评分相关（r=0.53，p<0.01）。这些研究结果表明，情绪刺激与人类尿液中胆红素氧化代谢产物的增加有关，生物吡咯烷可能是心理压力的有用标志物。基于这些关于心理应激、氧化应激和胆红素作为抗氧化剂之间可能的关系的报道，我们研究了心理应激导致氧化条件的可能性，以及随后胆红素与ROS反应产生的尿中生物吡啉浓度的增加。

Some authors have suggested that psychological stress induces the production of reactive oxygen species(ROS). Some studies have supported that bilirubin exerts anti-oxidative effects in vivo. However, it is not known whether ROS induced by psychological stress provoke bilirubin oxidation in vivo. We investigated if the concentration of biopyrrin, a bilirubin oxidative metabolite, increased in urine from subjects exposed to psychological stress. Sixty healthy male volunteers working in a pharmaceutical company were divided into a Group I which did not attend a conference, a Group II which attended a conference but did not deliver a speech, and a Group III which attended a conference and delivered speeches in the presence of the company executives. Subjective stress was scored (self-rating score) after subjects in Group III delivered their speeches at the conference. Urine was collected on the next day. The biopyrrin concentrations, as measured by enzyme-linked immunosorbent assay, were normalized to the urinary concentration of creatinine. The concentration of biopyrrin in Group III was significantly higher compared to that in Groups I and II (p<0.01 and p<0.05, respectively). Furthermore, in Group III, the concentration of biopyrrin correlated with the self-rating stress score (r=0.53, p<0.01). These findings suggest that emotional stimuli are associated with an increase in the oxidative metabolites of bilirubin in human urine, and that biopyrrins could be useful markers of psychological stress. Based on these reports on the possible relationship among psychological stress, oxidative stress and bilirubin as the antioxidant, we examined the possibility that psychological stress contributed to the oxidative conditions, and the subsequent increase of the urinary concentration of biopyrrins produced by the reaction of bilirubin with ROS.