Immunohistologial study on the expression of developmental intestinal epithelial antigens in embryogenesis and oncogenesis.

1.建立了可用于福尔马林固定组织染色的单克隆抗体，以研究大鼠胚胎和癌变肠上皮中发育抗原的表达。利用合成的寡肽，制备了小鼠抗蔗糖酶-异麦芽糖酶、MUC 2串联和非串联重复抗原、Cdx 1、Cdx 2和Musashi 1的单克隆抗体。2.利用新制备的单克隆抗体和固定的胚胎大鼠小肠组织，用免疫组化方法研究了发育肠上皮抗原的表达。Cdx 2在感染后11天开始在内胚层细胞核中表达，而Cdx 1在感染后15天才开始在内胚层细胞核中表达。随着小肠绒毛的发育，Cdx 1和Cdx 2分别趋向于定位于绒毛间内胚层和绒毛上皮。虽然MUC 2和蔗糖酶-异麦芽糖酶都是在出生前合成和表达的，但前者的表达先于后者的表达三天，后者的表达在pc第19天首次检测到。Cdxs的表达与MUC 2和蔗糖酶异构酶的表达不存在简单的相关性。3.研究了AOM诱导的大鼠小肠肿瘤中Apomucins、血型相关糖抗原和Cdx 2的表达。它们的表达在肿瘤中通常被抑制，尽管观察到MUC 5AC和H抗原在不正常表达它们的组织中表达。腺瘤和癌之间存在定量而非定性的表达差异。虽然近端结肠中表达的B抗原可能由MUC 5AC携带，但载脂粘蛋白、碳水化合物抗原和Cdx 2的表达之间没有明显的关联。Cdx 2的表达在AOM肿瘤中几乎完全被抑制。

1.Monoclonal antibodies that can be used for immunohistostaining of the formalin-fixed tissues were established to study the expression of the developmental antigens in rat embryonal and cancerous intestinal epithelia. Using the synthetic oligopeptides, mouse monoclonal antibodies against sucrase-isomaltase, MUC2 tandem and non-tandem repeat antigens, Cdx1, Cdx2, and Musashi1 were established.2.Expression of developmental intestinal epithelial antigens was studied immunohistochemically using the newly established monoclonal antibodies and the fixed tissues of embryonal rat intestines. Cdx2 was found to be expressed in the nuclei of endodermal cells at day 11 pc, but Cdx1 was first detected at day 15 pc. As the intestinal villi developed, Cdx1 and Cdx2, respectively, tended to localize in the intervillous endoderms and the villous epithelia. Although both of MUC2 and sucrase-isomaltase were synthesized and expressed before birth, the former expression preceded three days of the latter expression that was first detected at day 19 pc. The expression of Cdxs was not simply correlated with those of MUC2 and sucrase-isomlatase.3.Expression of apomucins, the blood group-related carbohydrate antigens and Cdx2 in the AOM-induced rat intestinal tumors was studied. Their expressions were generally suppressed in the tumors, though MUC5AC and H-antigen were observed to be expressed in the tissues not expressing them normally. Quantitative, but not qualitative, differences in the expression existed between the adenomas and the cancers. Although B-antigen expressed in the proximal colon might be carried by MUC5AC, there was no apparent association between the expressions of apomucins, the carbohydrate antigens and Cdx2. Expression of Cdx2 was almost completely suppressed in the AOM tumors.