The expression of HSP47 and the antisense therapy in the atherosclerosis animal model

动脉粥样硬化进展与热休克蛋白47的关系在ApoE基因敲除小鼠中作为动脉粥样硬化的动物模型进行了检验。观察动物动脉壁在5~28周的增龄过程中动脉粥样硬化的发生情况。22周后解剖显微镜下可见巨噬细胞聚集、动脉粥样硬化形成、泡沫细胞损伤。免疫组织化学染色检测ApoE基因敲除小鼠动脉壁中HSP47的表达(抗HSP47单抗：Stressgen Biotech公司，加拿大维多利亚)。ApoE基因敲除小鼠22周后，动脉粥样硬化病变处有明显的HSP47表达和I型胶原表达，ApoE基因敲除小鼠尾静脉注射针对HSP47的反义寡核苷酸可抑制动脉粥样硬化的进展，但该反义寡核苷酸的摄取不能有效地用于动脉粥样硬化的治疗。该模型中HSP47反义治疗的新策略。

The relationship between the progression of atherosclerosis and HSP47. was examined in ApoE knockout mouse as the animal model of atherosclerosis. The arterial walls of he animals were examined about the development of atherosclerosis at the aging ranging from 5 weeks to 28 weeks. Gross examination by dissection microscopy revealed the acculation of macrophage, the formation of atherom, foam cell lesion after 22 weeks mouse.The HSP47 expression in the arterial walls in the ApoE knockout mouse were assessed by immunostaining (Anti HSP 47 monoclonal antibody : Stressgen Biotechnolpgies Corp, Victoia BC, Canada). The HSP47 expression and the collagenI were evident in the arteriosclerotic lesions after 22weeks AopE knockout mouse.The antisense ODNs against HSP47 was administrated in the vein in the ApoE knockout mouse tail in order to suppress the progression of atherosclerosis.But the uptake of this antisense was not efficient for the purpose of atherosclerosis treatment in this animal model. The elnew strategy for the HSP47 antisense therapy in this model.