Plasma levels of ghrelin in anorexia nervosa patients

辛烷酰化的胃饥饿素（1-28）（完整的胃饥饿素）迅速降解为去辛烷酰形式或更小的片段（降解的胃饥饿素）。采用多种检测方法检测了30例神经性厌食症患者（体重指数：8.81-22.4 kg/m^2）和16例年龄匹配的健康女性血浆中完整和降解的胃饥饿素水平。通过免疫复合物转移酶免疫分析法（ICE-EIA）检测AN中特异性完整的胃饥饿素，血浆胃饥饿素水平低于对照组。通过活性胃饥饿素酶联免疫吸附试验（Active ghrelin ELISA Kit）检测的AN血浆胃饥饿素水平与对照组没有显著差异。在AN组中，Desacyl-Ghrelin ELISA检测的去辛酸型胃饥饿素、ghrelin Active RIA检测的n端胃饥饿素和ghrelin Total RIA检测的c端胃饥饿素水平均显著高于对照组，且与BMI呈显著负相关。AN患者血浆中完整胃饥饿素的水平从低于对照组到接近对照组不等。胃饥饿素的降解形式在AN中升高。

Octanoylated ghrelin (1-28) (intact ghrelin) is rapidly degraded to desoctanoyl forms or smaller fragments (degraded ghrelin). Plasma levels of intact and degraded ghrelin were examined in 30 anorexia nervosa patients (AN) (body mass index (BMI) : 8.81-22.4 kg/m^2) and 16 age-matched healthy women using several assay methods. Plasma ghrelin levels measured by immunocomplex transfer-enzyme immunoassay (ICE-EIA) detecting specifically intact ghrelin in AN were lower than those of controls. Plasma ghrelin levels in AN by Active Ghrelin ELISA Kit advertised as specifically detecting intact ghrelin were not significantly different from controls. Levels of desoctanoyl ghrelin by Desacyl-Ghrelin ELISA, N-terminus ghrelin by Ghrelin Active RIA, and C-terminus ghrelin by Ghrelin Total RIA were significantly higher in AN than in controls, and displayed significant negative correlations with BMI.Plasma levels of intact ghrelin in AN vary from lower than to similar to those of controls. Degraded forms of ghrelin are elevated in AN.