The role of liver in donor-specific immunotolerance

(1)在大鼠模型中，将ACI胸腺簇接种于Lewis大鼠皮下、静脉和门静脉。接种后7d， Lewis大鼠接受ACI心脏移植。无处理组移植后3 ~ 6天出现排斥反应，皮下接种缩短了生存时间，但静脉接种可延长生存时间1 ~ 3天，静脉接种可延长生存时间7天以上。然而，淋巴细胞接种与群集接种进入肝脏之间无显著差异。(2)在小鼠模型中，Balb/c胸腺簇分别接种于B6小鼠皮下、静脉和门静脉。接种后7d， B6小鼠与接种后的Balb/c小鼠进行混合淋巴细胞培养。皮下接种使MLR反应增敏，而静脉接种和p.v.接种抑制MLR反应。然而，淋巴细胞接种与群集接种进入肝脏之间无显著差异。(3)接种ACI胸腺簇后，Lewis大鼠各淋巴器官均未见微嵌合现象。(4)静脉注射链脲霉素诱导糖尿病。我们检查了一些使血糖正常化所必需的胰岛。结果表明，血糖正常化20IQ（胰岛当量）或更高。(5)将胰岛细胞和细胞簇同时注入门静脉，观察免疫耐受的获得情况。成批移植或单次移植均未见动物死亡。但同时注射可引起大鼠门脉栓塞和猝死。虽然我们尝试了各种实验方案，但任何实验程序都没有改善栓塞。

(1)In rat model, ACI thymus cluster was inoculated into subcutaneous, intravenous, and into portal vein of Lewis rats. 7days after inoculation, Lewis rats received ACI cardiac graft. No treated group rejected in 3 to 6 days after transplantation, subcutaneous inoculation shortened survival time, but i.v. inoculation prolonged survival for 1-3days, p.v. inoculation prolonged survival more than 7days. However, there is no significant difference between lymphocyte inoculation and cluster inoculation into the liver.(2)In mouse model, Balb/c thymus cluster was inoculated into subcutaneous, intravenous, and into portal vein of B6 mice. 7days after inoculation mixed lymphocyte culture was done between B6 mouse and inoculated Balb/c mouse. subcutaneous inoculation sensitized MLR reaction, but i.v. inoculation and p.v. inoculation suppressed MLR reaction. However, there is no significant difference between lymphocyte inoculation and cluster inoculation into the liver.(3)We could not find micro chimerism in any lymph organs of Lewis rat which was inoculated ACI thmus cluster into the liver.(4)Diabetes was induced by veneous injection of streptozotcin. We examined a number of islet necessary for normalizing he blood sugar. The results showed that the blood sugar normalized by 20IQ(islet equivalent) or more.(5)The islets and the cluster were injected into portal vein simultaneously, and the acquisition of immunotolerance was examined. The animals were hardly found to die in the clusters or ilets single transplantation. However, the simultaneous injection was found to cause portal embolism and sudden death of rats. Although we tried various experimental protocols, the embolism was not improved by any experimental procedures.