Cryopreservation of periosteum and whole bone by vitrification -establishment of organ freezing program.

组织和器官的低温保存在移植医学中可能发挥重要作用。本研究用DMSO作为低温保护剂，分别采用慢速（可编程生物冷冻机）和快速（玻璃化）冷冻方法，对从胚胎股骨中取出的鸡骨髓进行冷冻保存。解冻后，将其植入9天龄鸡胚的绒毛尿囊膜上，培养10天。通过软x线摄影（X-P）和组织学评估移植物的恢复和活力。X-Ps结果显示，经慢速冷却处理的移植物骨增厚，而质量略低于未冷冻对照。另一方面，移植物迅速冷却（玻璃化）萎缩。组织学结果显示，未冷冻对照组形成多层骨膜，外骨膜和扩孔腔处可见骨形成。慢速冷却后的移植物也有类似的结果，但玻璃化后的移植物没有骨形成。综上所述，DMSO慢速冷却比玻璃化冷却更适合保持鸡胚骨解冻后的活力。

Cryopreservation of tissues and organs may play an important role in transplantation medicine. In the present study, chick bone marrow which was reamed from embryonic femur was cryopreserved with DMSO as cryo-protectant by slow rate (programmable biological freezer) or rapid (vitrification) cooling procedures, respectively. After thawing, they were implanted on the chorioallantoic membrane of a 9^<th> day chick embryo, and cultured for 10 days. The grafts were recovered and viability evaluated by soft X-ray photography (X-P) and histologically. The findings of X-Ps showed that the graft processed by slow rate cooling gave hypertropbic bone, whereas the mass was slightly inferior to that of the un-frozen control. On the other hand, the grafts cooled rapidly (vitrification) were atrophied. The histological findings suggested that the un-frozen control gave multi-layered periosteum and bone formation at the external periosteum and reamed cavity were observed. The graft after slow rate cooling also gave similar findings, but those after vitrification showed no bone formation. Overall results indicated that slow rate cooling with DMSO was suitable to keep post thaw viability of chick embryonic bone rather than cooling by vitrifiction.