COMPREHEMSIVE ANALYSIS OF TUBULIN FUNCTIONAL GENE NETWORK IN YEAST

微管是细胞周期调控的关键机制之一，包括细胞生长、染色体分离等。微管的核心成分微管蛋白的调控涉及许多基因及其协同作用，但它们的功能网络尚不清楚。裂殖酵母在细胞周期调控因子的鉴定及其功能分析中发挥了重要作用。几种利用有丝分裂突变体的方法非常有用，许多基因被鉴定出来，它们的机制也变得清晰起来。我们构建了随机分离的裂殖酵母突变体库，并对单个突变基因及其之间的功能相互作用进行了系统分析。我们已经分离出1015株具有温度敏感突变而不是缺失突变的菌株，用于分析大部分必需基因。我们采取了两种不同的策略来分析微管蛋白调控网络。在一种方法中，我们研究了与分裂酵母中的微管结合蛋白dis1相互作用的基因。在第二种方法中，我们调查了微管蛋白及其相关基因的突变体。从这些分析中，我们发现了其中几个新的功能网络。这些结果有望阐明微管功能尚未分析过的多个相互作用的基因网络的存在。

Microtubule is one of the key machinery regulating cell cycle control, including cell growth, chromosome segregation. Although many genes and their cooperation are involved in regulation of tubulin, core component of microtubules, their functional networks are not known. Fission yeast Schizosaccharomyces pombe has played an important role for identification of cell cycle regulators and their functional analysis. Several approaches using mitotic mutant were extremely useful, and many genes were identified, and the mechanism of them became clear. We have constructed a randomly isolated mutant library of fission yeast and started systematic analysis about individual mutant genes and functional interaction among them. We have isolated 1015 strains having temperature sensitive mutation, not deletion mutation, for the purpose of analysis of most of essential genes. We have taken two kinds of different strategies to analyze tubulin regulatory network. In one approach, we investigated the genes interacting with dis1, a microtubule binding protein in fission yeast. In second approach we surveyed mutants of tubulin and their related genes. From these analysis we have found several new functional networks among them. These results are expected to elucidate the presence of multiple interactive gene networks that has not been analyzed in microtubule function.