Study for the detection of molecular target protein in cancer tissues by Dynamic Spot Method

我们开发了一个图像分析系统，用于检测2-DE凝胶中的特异性癌蛋白点。该系统可以分析银染凝胶图像在显影过程中的时间进程，区分癌组织和非癌组织的特异性蛋白质斑点，并开发了一种摄像系统，该系统包括用于显影操作的振荡系统和用于拍摄凝胶图像的间歇停止振荡系统。该系统的优点是无需人工操作，无需将凝胶从显影仪转移到摄像系统，图像分析与摄像系统相结合，可以分离重叠的斑点，因为我们可以从前一幅图像中得到原始分离斑点的信息，我们可以检测到非常微弱的斑点，因为该系统提供了后期图像，这些图像显示了相对清晰的少量蛋白质斑点。美国电泳学会

We developed an image analysis system for the detection of specific cancer protein spots in 2-DE gels. The system can analyze the time courses of silver stained gel images during developing processes, and distinguish the specific protein spots of cancer tissues from ones of non-cancer tissues.We also developed an camera system which include the shaking system for performing the developing operation and stop the shaking intermittently for taking picture of the gel images. The system has an advantage of the free hand operation for taking pictures, and we need not transfer the gel from developing instrument to a camera system.The combination of the image analysis and camera system can separate the overlapping spots, because we can get the information of original separated spots from the former images, and we can detect very faint spots because the system provide the later phase images which show the spots of only a few amount of protein relatively clear.These results were presented in 2004 Annual Meeting of the American Electrophoresis Society.