Discovery of Food Microorganism : Fast and Effective Degradation of Intestinal Lipoperoxide

本研究的目的是1）发现益生乳酸菌作为一种功能性食品，能够强烈地分解过氧化脂质，2）加速其在肠道中形成的降解能力。2004年，我们开发了分离具有过氧化脂质降解酶的乳酸菌的分离培养基。此外，我们还筛选出了具有较强过氧化氢降解能力的潜在菌株。在2005年从分离的乳酸菌中鉴定出有用的菌株，我们评估了过氧化物分解机制的降解能力。标准鉴定和鉴定：经过乳酸菌的基本鉴定和分组（乳酸菌手册，朝仓书店），我们筛选了48株可能适用于功能性食品的菌株，并从分离的菌株中排除了潜在的致病菌株。通过16 sDNA测序和分类学鉴定，对48株高脂过氧化物分解菌中的4株进行了全鉴定， ...更多信息 表型分析。作为鉴定的结果，它们被分类为植物乳杆菌科。过氧化脂质分解的评价：为了测量过氧化脂质的降解活性，我们制备了缺乏过氧化脂质和二价铁的乳杆菌标准GYP培养基，并在37 ℃下用需氧培养物调节。然后收获。我们利用亚油酸作为脂质过氧化物和枯烯过氧化物作为模型化合物来测量其活性。此外，使用过氧化氢作为亲水性底物。在分离的菌株中，植物乳杆菌Pan 1 -2表现出最高的过氧化脂质降解活性。这种独特的菌株完全降解过氧化枯烯，而对作为亲水底物的过氧化氢显示出约一半的降解活性。静息细胞的过氧化物降解机制：在过氧化丁基存在下，用于鉴定植物乳杆菌Pan 1 -2的反应产物，菌株表现出相等的过氧化丁基还原为丁醇。结果表明，该菌株具有过氧化物的2-电子还原。少

The objectives of this research are to 1) discover probiotic lactic acid bacteria as a functional food product enabling strong lipoperoxide breakdown, and 2) to accelerate its degrading capability being formed in the intestine.In 2004, we developed an isolation medium separated off lactic acid bacteria that has lipoperoxide degradative enzyme. In addition, we isolated potential strains that possess strong degrading capability of peroxide. In 2005 identifying useful strains from isolated lactic acid bacteria, we evaluated degrading capability of peroxide-decomposition mechanism.1. Standard Identification and Grouping : After basic identification and grouping of lactic acid bacteria (lactic acid bacteria Manual, Asakura Bookshop), we screened 48 strains potentially applicable for functional food product and excluded potential pathogenic strains from isolated strains. 4 out 48 strains performing high lipoperoxide decomposition were full-identified through 16sDNA Sequence and Taxonomy and … More Phenotypic Analysis. As a result of the identification, they are classified as a family of Lactobacillus plantarum.2. Evaluation of Lipoperoxide Decomposition : For measuring degradation activity of lipoperoxide, we prepared for Lactobacillus Standard GYP medium in lack of lipid peroxide and divalent iron, and conditioned with aerobic culture at 37? and then harvested. We utilized linoleic acid as a lipid peroxide and cumene peroxide as a model compound to measure its activity. In addition, hydrogen peroxide was used as a hydrophilic substrate. Among isolated strains, Lactobacillus plantarum Pan1-2 performed the highest degrading activity of lipoperoxide. This unique strain fully degrades cumene peroxide, while showing approximately a half degrading activity for hydrogen peroxide as a hydrophilic substrate.3. Peroxide Degradation Mechanism of Resting Cell : In presence of butyl peroxide for identifying reaction product of L.plantarum Pan1-2, the strain showed equal in reduction of butyl peroxide to butanol. As a result of that, it is suggested that the strain has 2-electron reduction of peroxide. Less