Development of a novel method to conserve genetic information of fish by cryopreservation of primordial germ cells

此前一直缺乏冷冻保存鱼类胚胎和卵子的方法。在这项研究中，我们第一次在低等脊椎动物中从冷冻保存的材料中再生了活的后代。我们的新方法可以成功地冷冻保存虹鲑鱼原始生殖细胞(PGC)。此外，通过移植到同种异体受体，冷冻保存的PGCs可以发育成功能卵子，并在与冷冻保存的精子受精后产生可存活的鱼苗。PGC超低温保存和移植将允许保存濒危鱼类物种的遗传信息。我们的系统也将是一个强大的工具，以保持驯化的鱼类品种携带商业价值的特征。

A method for cryopreserving fish embryos and eggs has previously been lacking. In this study, we have regenerated live offspring from cryopreserved materials for the first time in lower vertebrates. Our novel method allows the successful cryopreservation of rainbow trout primordial germ cells (PGCs). Moreover, through transplantation into allogenic recipients, the cryopreserved PGCs can develop into functional eggs and produce viable fry upon fertilization with cryopreserved spermatozoa. PGC cryopreservation and transplantation will allow the conservation of genetic information from endangered fish species. Our system will also be a powerful tool for maintaining domesticated fish strains carrying commercially valuable traits.