Molecularpharmacological analysis of mechanosensitive cation channels in vascular endothelial cells.

1)小鼠PKD 2L1（TRPP 2L1）的鉴定我们克隆了TRP通道家族成员之一的小鼠PKD 2L1基因。PKD2L1通过与PKD1相互作用在质膜上形成功能性阳离子通道（Murakami等人，J.Biol.Chem.，2005).2)TRPC的功能分析使用过表达转基因小鼠，阐明了神经元限制性沉默因子（NRSF）在TRPC 1表达中的调节作用（Ohba等人，2005).研究了乌诺前列酮对人主动脉平滑肌细胞中钙池操纵的Ca^2+内流（SOCE）的调节作用（Watanabe等人，2005).3)电压依赖性钙通道的功能作为阳离子通道家族成员，我们研究了电压依赖性钙通道的功能。Neuroscience letters，2004，Murakami等人，Brain Res.，2004年）。证明了β3亚基通过增加IP3产生对胰岛素分泌的重要性（Berggren等人，2004年）。评价了全氟辛烷磺酸（PFOS）和全氟辛酸（PFOA）对L型钙通道的抑制作用（Harada等人，2005年）。我们阐明了血栓素A2和前列腺素F2α参与炎症性心动过速（Takayama et al.，2005年）。

1)Identification of murine PKD2L1 (TRPP2L1)We cloned murine PKD2L1 gene, which is a member of TRP channel family. PKD2L1 forms functional cation channels on the plasma membrane by interacting with PKD1 (Murakami et al., J.Biol.Chem., 2005).2)Functional analysis of TRPCUsing overexpression transgenic mouse, regulatory role of neuron-restrictive silencing factor (NRSF) in expression of TRPC1 was clarified (Ohba et al., 2005).Regulatory effect of unoprostone on store operated Ca^<2+> entry (SOCE) in human aortic smooth muscle cells was investigated (Watanabe et al., 2005).3)Functions of voltage-dependent calcium channelsAs a cation channel family member, we investigated functions of voltage-dependent calcium channels.Roles of voltage- dependent calcium channels in nociceptive neurotransmission were analyzed (Murakami et al., Neuroscience letters, 2004, Murakami et al., Brain Res., 2004). Importance of β3 subunit on insulin secretion through increase in IP3 production was proved (Berggren, et al., 2004). Inhibitory effects of perfuorooctane sulfonate (PFOS) and perfuorooctanoate (PFOA) on L-type calcium channels were evaluated (Harada et al., 2005). We clarified involvement of Thromboxane A2 and prostaglandin F2α in inflammatory tachycardia (Takayama et al., 2005).