One donor-multiple recipients islet allotransplantation using two-layer method

实验1：在胰岛分离前用铺层法（TLM）保存胰腺，不仅提高了胰岛的产量，而且使胰岛的功能得到了很好的保持，几乎可以与新鲜分离的胰岛相媲美。实验2：采用TLM和腺苷消化法，消化后组织ATP水平保持良好。可以回收更多数量的胰岛。实验3：80 mmHg减压控制胶原酶注射（RPCI）可持续提高大鼠胰岛产量和体内功能;实验4：腹腔内充氧PFC可改善门静脉内胰岛移植的效果

Exp 1 : Tew layer method (TLM) for pancreas preservation prior to islet isolation resulted in excellent islet function in addition to improved islet yield, which is almost comparable to freshly isolated islets.Exp 2 : Tissue ATP levels after digestion were well maintained using TLM with adenosine digestion method. Greater numbers of islets could be retrieved. The new method was at least equivalent to islet function isolated by conventional method.Exp 3 : Reduced-pressure controlled collagenase injection (RPCI) at 80 mmHg could contribute to consistently high islet yield and in vivo function in a rat model.Exp 4 : intraperitoneal oxygenated PFC improves the outcome of intraportal islet transplantation