Functional Analyses of new genes involved in tooth germ development

我们利用cDNA减法对前期研究中新发现的与牙胚发育相关的基因功能进行了多种分析。结果是：1。胸腺酶P4 (TB4)：我们报道了mRNA在牙胚发育过程中的详细表达模式（见参考文献，Akhter, M et al.）。利用反义寡核苷酸抑制TB4 mRNA的器官培养系统提示TB4参与了牙胚形成的早期发育。酵母双杂交实验发现，TB4.2的伴侣蛋白包括肌动蛋白结合蛋白、MAP激酶信号转导蛋白、脂蛋白结合蛋白、DNA修复蛋白、转录调节蛋白等。磷酸甘油酸激酶1 (Phosphoglycerate kinase 1, Pgk1)：原位杂交研究表明，Pgk1是糖酵解系统中的一种酶，与gapdh形成复合物，在牙胚发育中起重要作用。set-α - 4：酵母双杂交研究表明，一种转录调节因子、一种泛素系统相关的酶等可能是set-α - 4的伴侣蛋白。一种新的细胞粘附因子样蛋白：这种假设的蛋白属于免疫球蛋白超家族。酵母双杂交研究表明，肌动蛋白结合蛋白、凝集素相关蛋白、细胞外基质相关蛋白、信号转导蛋白、钙粘蛋白相关蛋白等作为功能伴侣蛋白。此外，我们发表了另一篇与II/III型牙病Run×2/Cbfal和牙齿发育相关的研究（参见参考文献，Kobayashi等人）。

We have performed the various kinds of analyses for elucidating the functions of genes involved in tooth germ development, which were newly found in our previous study using cDNA subtraction methods. The results are:1. thymosin P4 (TB4): The detailed expression patterns of mRNA in tooth germ development are reported in our paper (See references, Akhter, M et al.). The organ culture system using antisense-oligonucleotide against TB4 mRNA suggested the TB4 is involved in early development of tooth germ formation. The yeast two hybrid experiment revealed an actin-binding protein, a MAP kinase signal transduction protein, a lipoprotein-binding protein, a protein involved in DNA repair, a transcriptional regulator, as the partner proteins of TB4.2. Phosphoglycerate kinase 1 (Pgk1): In situ hybridization study showed that Pgk1, an enzyme in glycolytic system, play an important role in tooth germ development, in a complex with GAPDH.3. Set-a: The yeast two hybrid study suggested a transcriptional regulator, an enzyme associated with ubiquitin system, etc. may be partner proteins for set-α.4. A novel cell-adhesion factor-like protein: This hypothetical protein belongs to Immunoglobulin superfamily. The yeast two hybrid study suggested that an actin-binding protein, a lectin associated protein, an extracellular matrix associated protein, a signal transduction protein, a protein associated with cadherin, etc. as the functional partner proteins.Furthermore, we published another work associated with type II/III Run×2/Cbfal and tooth development (See references, Kobayashi et al.)