Establishment of bone invasion model by oral cancer and effects of NF-kB inhibitor on bone invasion.

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Nuclear factor-kB (NF-kB) is constitutively activated in many cancers, including oral squamous cell carcinoma (OSCC). However, the cellular mechanism underlying NF-kB's promotion of bone invasion by OSCC is unclear. Therefore, we investigated the role of NF-kB in bone invasion by OSCC in vivo. Immunohistochemical staining of OSCC invading bone in 10 patients indicated that the nuclear translocation of NF-kB was increased in OSCC compared with normal squamous epithelial cells. We next injected mouse OSCC SCCVII cells into the masseter region of C3H/HeN mice. Treatment with NBD peptide, a selective NF-kB inhibitor, decreased zygoma and mandible destruction by SCCVII cells, reduced number of osteoclasts by inhibiting RANKL expression in osteoblastic cells and SCCVII cells, increased apoptosis and suppressed the proliferation of SCCVII cells. Taken together, our data clearly indicate that inhibition of NF-kB is useful for inhibiting bone invasion by OSCC