Control of salivary secretion through gap junctional communication

自主神经系统调节唾液腺的分泌功能，在唾液腺中缝隙连接细胞通讯发达。1细胞通讯抑制剂抑制腮腺组织条中自主神经激动剂对淀粉酶的释放。2细胞通讯抑制剂抑制颌下腺组织条中过氧化物酶的分泌。3细胞通讯抑制剂诱导某些腺泡细胞的胞吐形态，并增强与液体分泌相关的形态，但不改变受刺激的导管形态。4细胞通讯抑制剂抑制某些腺泡细胞内[Ca~(2+)]i的升高。虽然钙波在许多腺泡细胞中传播。5细胞松弛素抑制组织条中淀粉酶的分泌和液体分泌相关的形态，但多种类型的激酶抑制剂对其抑制作用不明显。6毛细血管网络环绕一组与导管相连的腺泡。腺泡细胞表面很少见到神经末梢。7个腺泡细胞簇的振荡分泌电流表现出几种不同的强度，细胞通讯抑制剂处理后产生均匀的强度电流。缝隙连接细胞通讯通过信号传递促进唾液分泌，补偿神经支配的罕见分布。

The autonomic nerve system regulates the secretory function of the salivary gland in which gap junctional cell communication is well developed. We investigated the control mechanism of secretory function through the cell communication, and obtained the following results.1 Cell communication inhibitor suppressed amylase release with autonomic nervous agonists in tissue strips of the parotid.2 Cell communication inhibitor suppressed peroxidase secretion with the field electrical stimulation in the tissue strips of the submandibular glands.3 Cell communication inhibitor induced exocytotic morphology in some acinar cells and enhanced fluid secretion-related morphology, but it did not change the stimulated duct morphology.4 Cell communication inhibitor restricted the increase in [Ca2+]i in some acinar cells, although calcium wave is propagated in many acinar cells.5 The amylase secretion and fluid secretion-related morphology in the tissue strips was suppressed with cytochalasin, but not markedly with many types of kinase inhibitors.6 Capillary network encircled a group of acini connected with the ducts. Nerve terminals were rarely observed in the surface of the acinar cells.7 Oscillatory secretion currents in clumps of acinar cells exhibited a few kinds of intensity, and cell communication inhibitor treatment induced the uniform intensity currents.Gap junctional cell communication contributes to the salivary secretion through signal transfer compensated the rare distribution of nerve innervation.