Regulation of bicarbonate secretion as fluid excretion in the salivary ducts

唾液管中碳酸氢盐分泌作为液体排泄的调节

• 批准号: 12671808
• 负责人: SHIBA Yoshiki
• 金额: $ 2.11万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671808/
• 关键词: Duct secretion; Salivary secretion; ion channel; CFTR; bicarbonate; bicarbonate secretion

In the present study, we investigated the exclusion of fluorescent dye from the duct lumen by carbachol (CCh) in isolated parotid interlobular duct segments to clarify the ability of the ducts for the fluid secretion with bicarbonate secretion. CCh decreased the fluorescent intensity in the duct lumen during the superfusion of the fluorescent dye, and CCh flushed out small viscous substances stained with the fluorescent dye from isolated duct lumen. Methazolamide and removal of external Ca^<2+> suppressed CCh-induced clearance of the duct fluorescence. A major portion was inhibited by removal of bicarbonate in the superfusion solution and DPC, but not low concentration of external CT, bumetanide or DIDS. Patch-clump investigation indicated the presence of Ca^<2+> -activated anion channels, and gramieidin-perforated patched analysis indicated that bicarbonate was a major portion of outward currents in the isolated duct cells. These results suggest that carbachol evoles fluid secretion possibly through Ca^<2+> -activated, DPC-sensitive anion channels with HCO_3^- secretion in the rat parotid intralobular ducts.

在本研究中，我们研究了在分离的腮腺小叶间导管段中卡巴胆碱（CCh）从导管腔中排除荧光染料的情况，以阐明导管通过碳酸氢盐分泌进行液体分泌的能力。在荧光染料灌注过程中，CCh 降低了导管腔内的荧光强度，并且 CCh 从孤立的导管腔中冲出了被荧光染料染色的小粘性物质。醋甲唑胺和外部Ca 2+ 的去除抑制了CCh诱导的导管荧光清除。大部分被灌流溶液和DPC中碳酸氢盐的去除所抑制，但低浓度的外部CT、布美他尼或DIDS则不受抑制。斑块丛研究表明存在Ca 2+ 激活的阴离子通道，而短杆菌穿孔斑块分析表明碳酸氢根是分离的导管细胞中外向电流的主要部分。这些结果表明，卡巴胆碱可能通过大鼠腮腺小叶内导管中的Ca 2+ 激活的、DPC敏感的阴离子通道和HCO_3- 分泌来产生液体分泌。

项目成果

Hirono C, Nakamoto T, Sugita M, Iwasa Y, Akagawa A, and Shiba Y: "Gramicidin-perforated patch analysis on HCO3-secretion through a forskolin-activated anion channel in rat parotid intralobular duct cells"J. Membrane Biol. 180. 11-19 (2001)

Hirono C、Nakamoto T、Sugita M、Iwasa Y、Akakawa A 和 Shiba Y：“通过大鼠腮腺小叶内导管细胞中毛喉素激活的阴离子通道对 HCO3 分泌进行短杆菌肽穿孔贴片分析”J。

Hirono, C., Nakamoto, T., Sugita, M., Iwasa, Y., Akagawa, A., Shiba, Y.: "Gramicidin-perforated patched analysis on HCO3-secretion through a forskolin-activated anion channel in rat parotid intralobular duct cells"J.membrane Biol.. 180. 11-19 (2001)

Hirono, C.、Nakamoto, T.、Sugita, M.、Iwasa, Y.、Akakawa, A.、Shiba, Y.：“通过大鼠腮腺毛喉素激活的阴离子通道对 HCO3 分泌进行短杆菌肽穿孔补丁分析

Hirnono, C., Nakamoto, T., Sugita, M., Iwasa, Y., Akagawa, A., Shiba, Y.: "Gramicidin-perforated patched analysis on HCO3-secretion through a forskolin-activated anion channel in rat parotid intralobular duct cells."J. membrane Biol.. 180. 11-19 (2001)

Hirnono, C.、Nakamoto, T.、Sugita, M.、Iwasa, Y.、Akakawa, A.、Shiba, Y.：“通过大鼠腮腺毛喉素激活的阴离子通道对 HCO3 分泌进行短杆菌肽穿孔补丁分析

Hirono,C.,Nakamoto,T.,Sugita,M.,Iwasa,Y.,Akagawa,A.and Shiba,Y.: "Gramicidin-perforated patch analysis on HCO3- secretion through a forskolin-activated anion channel in rat parotid intralobular duct cells."J.Membrane Biol.. 180(In press). (2001)

Hirono,C.、Nakamoto,T.、Sugita,M.、Iwasa,Y.、Akakawa,A. 和 Shiba,Y.：“通过大鼠腮腺毛喉素激活的阴离子通道对 HCO3 分泌进行短杆菌肽穿孔贴片分析