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Regulation with secretory signals and neurotrophic factors of salivary secretion-related protein expression

分泌信号和神经营养因子对唾液分泌相关蛋白表达的调节

基本信息

批准号：
18390492
负责人：
SHIBA Yoshiki
金额：
$ 10.38万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390492/
关键词：
oral science salivary secretion secreted protein gene expression hormone 遺伝子発現唾液腺分泌

项目摘要

Secretory protein contents in the salivary glands are regulated with various factors. We investigated the expression mechanism of secretory proteins with secretory stimulation and autonervous system, and obtained the following results.1 Proteins in the salivary glands were modulated with isoproterenol (ISP) application, but not markedly with carbachol (CCh) and dexamethasone.2 Treatment of isolated salivary tissues with ISP induced the expression of c/EBPs.3 Culture of salivary acinar cells induced the degenerative changes, and we could not identified the contribution of cell communication to the protein expression control in cultured cells.4 Denervation did not induce marked changes in salivary gland weight and amylase content, but further treatment with ISP induced the tissue weight and new protein expression. ISP decreased amylase content but increased their mRNA levels. ISP increased peroxidase content, and denervation decreased it5 ISP decreased Akt, mTOR, S6k, and p27, but increased PCNA. Denervation tended to decrease S6k, but did not any changes in other proteins. ISP and denervation decreased S6k and p27, but increased PCNA.6 Rapamycin (Rap), blocker of mTOR, potentiated the decrease in the gland weight. Rap decreased peroxidase content, but not amylase content. Salivary protein expression might be regulated through Akt-mTOR-S6k signaling system with secretory and autonomic nervous stimulation.

唾液腺中分泌蛋白的含量受多种因素的调节。本研究从分泌刺激和自主神经系统两个方面探讨了唾液腺分泌蛋白的表达机制，得到以下结果：1异丙肾上腺素（ISP）对唾液腺分泌蛋白的表达有明显的调节作用，而卡巴胆碱（CCh）和地塞米松对唾液腺分泌蛋白的调节作用不明显; 2 ISP处理离体唾液腺组织可诱导c/EBP的表达; 3培养的唾液腺腺细胞可诱导c/EBP的退行性改变，并且我们不能确定细胞通讯对培养细胞中蛋白表达控制的贡献。4去神经支配没有引起唾液腺重量和淀粉酶含量的显著变化，但是进一步用ISP处理诱导了组织重量和新的蛋白表达。ISP降低淀粉酶含量，但提高mRNA水平。ISP使Akt、mTOR、S6 k和p27蛋白表达降低，但使PCNA表达升高。失神经支配有降低S6 k的趋势，但对其他蛋白质无影响。ISP和去神经支配降低了S6 k和p27，但增加了PCNA。6雷帕霉素（Rap），mTOR的阻断剂，加强了腺体重量的减少。油菜降低过氧化物酶含量，但不影响淀粉酶含量。唾液蛋白的表达可能通过Akt-mTOR-S6 k信号系统在分泌和自主神经刺激下进行调节。