A Genetic Engineering Approach which Permits Immediate Loading of Dental Implant

牙种植作为一种重要的治疗方式，由于其良好的可预见性而备受关注。为达到种植体在骨内的稳定，骨愈合需要3 ~ 6个月。如果这个时间可以缩短，可能对医生和病人都有益。近年来，即刻加载法已被引入种植界，然而，这种治疗方法的科学依据很少。在本研究中，我们假设立即加载是安全的，没有任何并发症，我们打算阐明骨愈合与加载时间和数量之间的关系。2004年，我们使用有限元法研究了适当的加载方法以及力如何扩散到周围的骨骼。根据这些结果，我们得到了几个数量的犬齿，并进行了拔牙。2005年，我们对加载的种植体周围进行了组织学分析，然后使用微阵列方法分析了加载种植体附近骨周围的蛋白质表达模式。将两颗种植体置入犬齿下颌骨，利用快速膨胀螺钉在种植体间施加静载荷进行正畸。加载7天后，植入物周围的骨在没有破骨细胞存在的情况下被破坏。在微阵列分析中，一些蛋白质的mRNA表达模式随着负载的变化而改变。提示该蛋白表达的改变可能在骨整合障碍中起作用。

Dental implantology attracts attention as an important treatment modality for edentulism because of its excellent predictability. To achieve the stability of implant in the bone, 3 to 6months are required for the bone healing. If this duration can be truncated, it may be beneficial for both doctors and patients. Recently, immediate loading procedure has been introduced into the implantology, however, there are few scientific evidences for such treatment.In the present study, we hypothesized that immediate loading is safely performed, without any complication, and we intended to elucidate the relationship between bone healing and the timing and quantity of the loading. In 2004, we examined the appropriate method for the loading and how the force spread into the surrounding bone using finite element method. Based upon these results, we obtained several numbers of canines and teeth extraction was performed. In 2005, we performed the histological analysis around the loaded dental implants and then we analyzed the protein expression pattern around the bone adjacent to the loaded implant, using microarray method. Two implants were installed into canine mandible, and then static load was operated between implants using rapid expansion screw for orthodontics. Seven days after the load, the bone around the implant was disrupted without the presence of osteoclasts. In microarray analyses, some kinds of proteins revealed the alteration of mRNA expression pattern in response to the loading. It was suggested that the alteration of the protein expression might play a role in the disturbance of osseointegration.