Novel coated nanostructured implant surfaces to increase osseointegration and decrease peri-implantitis in a physiologic rat model

项目摘要/摘要： R01竞争重新提交更新的重点是建立纳米结构技术的有效性 具有保护性涂层，可增加骨整合，最大限度地减少细菌增殖和腐蚀 围绕着钛牙科植入物。这是我们目前拨款的延续，我们开发和优化了一个 用于在陶瓷上进行防腐涂层，目的是生产抗断裂修复体。我们的数据 优化后的硅具有优异的耐腐蚀性、抗菌性能和成骨性能 碳化物(碳化硅)涂层和这一竞争更新将为这种涂层在牙科领域提供更广泛的应用 植入物。这项研究的长期目标是开发一种植入体，它将增加骨整合和 预防种植体周围炎，以改善长期修复效果。总体目标是批判性地评估 牙种植体的防腐、抗菌和骨整合性能具有开创性的设计 将包括我们在之前的资金中开发的纳米结构形貌和保护涂层 周期，使用将生理模拟种植周围炎的病因学的大鼠模型。 这一独特的转译研究项目将使用生理诱导的大鼠模型。 细菌介导的种植体周围炎。通过阳极氧化将纳米结构技术结合在一起 钛表面，专为骨功能和抗菌功效而定制，将与 在上一个供资周期中开发了保护性涂层(碳化硅和碳化硅)。 这种组合有望增强骨整合、抗菌和防腐性能。 这两种表面修饰都能创造一种可预测的种植修复。将进行初步的体外实验 确定理想的骨整合纳米结构表面。我们将把这些定制的纳米结构涂覆到 开发一种新的种植体设计，用于动物模型的植入，以确定骨整合、抗菌和 利用最先进的纳米CT可视化技术研究种植体周围炎的体内发病机制的防腐性能。 我们提出以下目标来验证我们的中心假设：目标1：优化纳米结构设计 增加钛表面的骨整合能力；目标2：优化定制化的效果 纳米结构涂层植入物表面对细菌黏附和增殖以及体外腐蚀的影响；目标3： 在体测定制化纳米结构种植体骨结合的速度和数量 应用纳米CT评价兔种植体植入早期和晚期的形态 评估和组织分析；目的4：确定定制的纳米结构涂层的有效性 种植体骨结合及体内多菌体种植体周围炎的预防 种植炎，它在生理上模仿人类的种植周围炎。

PROJECT SUMMARY/ABSTRACT: This R01 competing resubmission renewal is focused on establishing the efficacy of nanostructure technology with a protective coating in increasing osseointegration, and minimizing bacterial proliferation and corrosion around titanium dental implants. This is a continuation of our current grant, which developed and optimized a coating for corrosion resistance on ceramic with the goal of producing fracture-resistant restorations. Our data demonstrated excellent corrosion resistance, anti-bacterial and bone forming properties of an optimized silicon carbide (SiC) coating and this competing renewal will provide an expanded application for this coating with dental implants. The long-term goal of this research is to develop an implant which will increase osseointegration and prevent peri-implantitis to improve long-term restoration outcomes. The overall objective is to critically evaluate the anti-corrosive, anti-bacterial and bone integration properties of dental implants with a pioneering design that will incorporate nanostructured topography and protective coatings, which we developed in the previous funding cycle, using a rat model that will physiologically simulate the etiology of peri-implantitis. This unique, translational research project will employ the use of a rat model with physiologically induced bacterial-mediated peri-implantitis. A combination of nanostructure technology through anodization of the titanium surface, customized for osteofunctional and anti-bacterial efficacy, will be applied in conjunction with protective coatings (Silicon Carbide and Quaternized Silicon Carbide) developed during the last funding cycle. This combination is expected to potentiate the osseointegrative, anti-bacterial and anti-corrosive properties of both surface modifications to create a predictable implant restoration. Initial in vitro experiments will be conducted to determine ideal nanostructure surface for osseointegration. We will coat these customized nanostructures to develop a new implant design for placement in animal models to determine osseointegration, anti-bacterial and anti-corrosive properties using state of the art nanoCT visualization of the in vivo pathogenesis of peri-implantitis. We propose the following aims to test our central hypothesis: Aim 1: To optimize nanostructure design for increasing osseointegrative potential on Ti surfaces in vitro; Aim 2: To optimize the effect of customized nanostructured coated implant surfaces on bacterial adhesion and proliferation, and corrosion in vitro; Aim 3: To determine in vivo the rate and amount of osseointegration in implants with customized nanostructure topography during early and late stage implant placement in rabbits using stability evaluation, nano-CT evaluation and histological analysis; Aim 4: To determine the effectiveness of customized nanostructured coated implants in osseointegration and prevention of peri-implantitis in vivo in the rat model of polymicrobial peri- implantitis, which physiologically mimics human peri-implantitis.