Novel coated nanostructured implant surfaces to increase osseointegration and decrease peri-implantitis in a physiologic rat model

新型涂层纳米结构种植体表面可增加生理大鼠模型中的骨整合并减少种植体周围炎

• 批准号: 10645782
• 负责人: Josephine F. Esquivel-Upshaw
• 金额: $ 65万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-21 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10645782
• 关键词: Address; Adhesions; Animal Model; Anodes; Anti-Bacterial Agents; Apoptosis; Bacteria; Bacterial Adhesion; Bone Growth; Bone Tissue; Cell Wall; Cells; Ceramics; Cervical; Charge; Chemicals; Consensus; Corrosion; Corrosives; Custom; Data; Dental; Dental Implants; Dental crowns; Deposition; Development; Diabetes Mellitus; Disease; Disease Progression; Educational workshop; Effectiveness; Etiology; Europe; Evaluation; Extravasation; Failure; Fracture; Funding; Future; Goals; Grant; Histologic; Human; Implant; Implantation procedure; In Vitro; Incidence; Industry; Infection; Inflammation; Inflammatory; Investments; Lead; Link; Maintenance; Mediating; Modality; Modeling; Modification; Mucositis; Mucous Membrane; Nanostructures; Nitrogen; Oral health; Oryctolagus cuniculus; Osseointegration; Osteoblasts; Osteogenesis; Outcome; Outcome Study; Oxides; Pathogenesis; Patients; Periodontal Diseases; Periodontitis; Physiological; Plasma Enhancement; Positioning Attribute; Prevalence; Prevention; Prevention strategy; Prognosis; Proliferating; Property; Prosthesis; Public Health; Rattus; Reaction; Recording of previous events; Recurrence; Regimen; Research; Research Project Grants; Residual state; Resistance; Risk Factors; Role; Smoking; Structure; Surface; Technology; Testing; Tissues; Titanium; Tooth structure; Translational Research; United States; Visualization; alveolar bone; bone; bone loss; cost; design; effective therapy; effectiveness evaluation; experimental study; implant design; improved; in vivo; keratinization; microbial; microbiome; nano; novel; osteoblast proliferation; particle; peri-implantitis; prevent; restoration; silicon carbide; soft tissue; standard of care; success; vapor

PROJECT SUMMARY/ABSTRACT: This R01 competing resubmission renewal is focused on establishing the efficacy of nanostructure technology with a protective coating in increasing osseointegration, and minimizing bacterial proliferation and corrosion around titanium dental implants. This is a continuation of our current grant, which developed and optimized a coating for corrosion resistance on ceramic with the goal of producing fracture-resistant restorations. Our data demonstrated excellent corrosion resistance, anti-bacterial and bone forming properties of an optimized silicon carbide (SiC) coating and this competing renewal will provide an expanded application for this coating with dental implants. The long-term goal of this research is to develop an implant which will increase osseointegration and prevent peri-implantitis to improve long-term restoration outcomes. The overall objective is to critically evaluate the anti-corrosive, anti-bacterial and bone integration properties of dental implants with a pioneering design that will incorporate nanostructured topography and protective coatings, which we developed in the previous funding cycle, using a rat model that will physiologically simulate the etiology of peri-implantitis. This unique, translational research project will employ the use of a rat model with physiologically induced bacterial-mediated peri-implantitis. A combination of nanostructure technology through anodization of the titanium surface, customized for osteofunctional and anti-bacterial efficacy, will be applied in conjunction with protective coatings (Silicon Carbide and Quaternized Silicon Carbide) developed during the last funding cycle. This combination is expected to potentiate the osseointegrative, anti-bacterial and anti-corrosive properties of both surface modifications to create a predictable implant restoration. Initial in vitro experiments will be conducted to determine ideal nanostructure surface for osseointegration. We will coat these customized nanostructures to develop a new implant design for placement in animal models to determine osseointegration, anti-bacterial and anti-corrosive properties using state of the art nanoCT visualization of the in vivo pathogenesis of peri-implantitis. We propose the following aims to test our central hypothesis: Aim 1: To optimize nanostructure design for increasing osseointegrative potential on Ti surfaces in vitro; Aim 2: To optimize the effect of customized nanostructured coated implant surfaces on bacterial adhesion and proliferation, and corrosion in vitro; Aim 3: To determine in vivo the rate and amount of osseointegration in implants with customized nanostructure topography during early and late stage implant placement in rabbits using stability evaluation, nano-CT evaluation and histological analysis; Aim 4: To determine the effectiveness of customized nanostructured coated implants in osseointegration and prevention of peri-implantitis in vivo in the rat model of polymicrobial peri- implantitis, which physiologically mimics human peri-implantitis.

项目概要/摘要： R01 竞争性重新提交更新的重点是确定纳米结构技术的功效 具有保护涂层，可增加骨整合，并最大限度地减少细菌增殖和腐蚀 钛合金牙种植体周围。这是我们当前赠款的延续，该赠款开发并优化了 陶瓷上的耐腐蚀涂层，目的是生产抗断裂修复体。我们的数据 表现出优化硅的优异耐腐蚀性、抗菌性和成骨特性 硬质合金 (SiC) 涂层和这种竞争性的更新将为这种涂层在牙科领域的应用提供扩展 植入物。这项研究的长期目标是开发一种能够增加骨整合和 预防种植体周围炎，改善长期修复效果。总体目标是批判性地评估 具有开创性设计的牙种植体的防腐、抗菌和骨整合特性 将结合我们在上一次融资中开发的纳米结构形貌和保护涂层 循环，使用大鼠模型在生理上模拟种植体周围炎的病因。 这个独特的转化研究项目将采用生理诱导的大鼠模型 细菌介导的种植体周围炎。通过阳极氧化结合纳米结构技术 专为骨功能和抗菌功效而定制的钛表面将与 在上一个融资周期中开发的保护涂层（碳化硅和季铵化碳化硅）。 这种组合有望增强骨整合、抗菌和防腐特性 两种表面修饰都可实现可预测的种植体修复。将进行初步体外实验 确定骨整合的理想纳米结构表面。我们将把这些定制的纳米结构涂覆到 开发一种新的植入物设计，用于放置在动物模型中，以确定骨整合、抗菌和 使用最先进的纳米 CT 可视化种植体周围炎的体内发病机制来实现防腐蚀性能。 我们提出以下目标来检验我们的中心假设： 目标 1：优化纳米结构设计 体外增加钛表面的骨整合潜力；目标2：优化定制效果 纳米结构涂层植入物表面对细菌粘附和增殖以及体外腐蚀的影响；目标 3： 确定具有定制纳米结构的植入物体内骨整合的速率和数量 使用稳定性评估、纳米 CT 评估兔子早期和晚期种植体植入过程中的地形 评估和组织学分析；目标 4：确定定制纳米结构涂层的有效性 种植体在多种微生物周围微生物模型大鼠体内的骨整合和预防种植体周围炎的作用 种植体炎，其生理学类似于人类种植体周围炎。

项目成果

Properties of SiCN Films Relevant to Dental Implant Applications.

• DOI: 10.3390/ma16155318
• 发表时间: 2023-07-28
• 期刊: MATERIALS
• 影响因子: 3.4
• 作者: Xia, Xinyi;Chiang, Chao-Ching;Gopalakrishnan, Sarathy K.;Kulkarni, Aniruddha V.;Ren, Fan;Ziegler, Kirk J.;Esquivel-Upshaw, Josephine F.
• 通讯作者: Esquivel-Upshaw, Josephine F.

Enhancing the Hydrophobicity and Antibacterial Properties of SiCN-Coated Surfaces with Quaternization to Address Peri-Implantitis.

• DOI: 10.3390/ma16175751
• 发表时间: 2023-08-22
• 期刊: Materials (Basel, Switzerland)