Influence of Missing Molar Teeth (SDA) on Stress Hormone in Saliva

本研究旨在探讨磨牙缺失与应激激素的关系。研究对象为25例单侧或双侧磨牙缺失患者，其中女性18例，男性7例，平均年龄62.1岁。将患者分成5组，由5名牙医进行义齿修复。所有受试者在牙科椅子上以直立姿势休息，并要求咀嚼棉卷(唾液)2min，以采集唾液样本。分别于就诊后、就诊前、就诊后即刻、就诊后即刻、就诊前、就诊后及就诊后每次就诊时、治疗前、治疗后即刻采集唾液，于测定前立即冰冻-50℃保存，用ELISA试剂盒检测皮质醇和嗜铬粒蛋白A。采用重复测量的单因素方差分析方法分析RPD治疗前后的比较。结论：1.首次就诊时RPD患者治疗前唾液皮质醇和嗜铬粒体素浓度明显高于治疗后。患者戴用可摘局部义齿并对义齿满意时，唾液皮质醇和嗜铬粒蛋白A浓度较其他义齿时显著降低。患者摘除RPD 3d后，唾液皮质醇和嗜铬粒蛋白A浓度显著升高，提示SDA是应激源之一，RPD的治疗应减轻应激。然而，由于这些结果可能涉及许多其他因素，因此需要一个新的实验模型来进行分析。

The purpose of this study is to elucidated relationship between missing molar teeth and the stresses hormone.Twenty-five patients (18 females and 7 males, average 62.1 year-old) whose unilateral or bilateral molar teeth were missing (Shortened Dental Arch 'SDA') were participated in this study. They were divided into 5 groups and were treated their denture by 5 dentists. All subjects were rested in an upright position on the dental chair, and asked to chew a cotton roll (salivette) for 2 min, which was to collect their saliva sample. The stages of saliva collection were immediately after interviewing, just before treatment, immediately after treatment, and before leaving hospital on the first visit, and at each subsequent visit, before and after treatment.Saliva samples were immediately frozen-50℃ for storage prior to assay, and cortisol and chromogranin A was measured by ELISA kit. Comparisons between before and after treatment of RPD were analyzed using an ANOVA with repeated measures.1. The concentrations of free salivary cortisol and chromogranin before treatment for the RPD at the first time of visit were significantly higher than those after treatment.2. When the patients were provided the removable partial denture (RPD) and satisfied with their denture, the concentration of salivary cortisol and chromogranin A decreased significantly compare with those at the other visits.3. After the patients removed their RPD for 3 days, the concentration of salivary cortisol and chromogranin A increase significantly.The present data suggest that SDA was a factor for a source of stress and that treatment of RPD should reduce the stress. However, as these results might involve many other factors, a new experimental model should be required for the analysis.