Feeding behavior and growth in Chicks treated RNA interference

(1)RNAi的体外效应：我们发现RNAi处理暂时抑制COS-7细胞中chLEPR mRNA和蛋白的表达。在CHO-chLEPR细胞株中，RNAi处理组的荧光素酶活性在瘦素负载（125 ng/ml）后3小时显著低于对照组，但不低于24小时。实验结果表明，设计的RNAi能有效抑制瘦素受体的信号传导。(2)体内实验结果：注射后15、24 h，所设计的RNAi对鸡间脑chLEPR mRNA的表达无影响。与表达相似，注射对雏鸡的采食量和体重增加均无影响。(3)瘦素或NPY Y1受体反义寡核苷酸的作用（体内）：与RNAi类似，瘦素受体反义寡核苷酸不影响chLEPR mRNA的表达，也不影响雏鸡的采食量和增重。另一方面，注射后15小时，NPY Y1受体的反义寡核苷酸抑制受体mRNA的表达。

(1) Effects of RNAi in vitro: We found that RNAi treatments temporarily inhibited the expression of chLEPR mRNA and the protein in COS-7 cells. In CHO-chLEPR cell strains, the luciferase activity of RNAi treated group was significantly lower than that of control at 3, but not 24 hr after leptin loading (125 ng/ml). It seemed that the designed RNAi was effective to inhibition of the signaling transmission in the leptin receptors.(2) Effects of RNAi in vivo: The designed RNAi did not affect the expression of chLEPR mRNA in diencephalon of chicks at 15 or 24 hr after injection. Similar to the expression, the injection did not influence on both feed intake and body weight gain in chicks.(3) Effect of anti-sense oligonucleotide for leptin or NPY Y1 receptor (in vivo) : Similar to RNAi, anti-sense oligonucleotide for leptin receptor did not affect the expression of chLEPR mRNA, and feed intake and body weight gain in chicks. On the other hand, anti-sense oligonucleotide for NPY Y1 receptor suppressed the expression of the receptor mRNA at 15 hr after injection.