Analysis of the bacterial flora for the zero emission oriented toilet system and improvement of the clarification function

实验室规模的模型零排放导向厕所系统与人类粪便运行，直到2005年。2006年，厕所系统使用由蛋白胨、酵母提取物、纤维素和NaCl组成的人工污水，而不是人粪便，因为人粪便的成分每天都在变化。并将木屑或海绵块作为细菌载体包装在处理槽中。结果表明，木屑和海绵块中BOD的去除率分别为83%和67%。木屑是必须补充的，因为木屑被细菌消化了。2006年，将处理池改造为好氧池和厌氧池两级，并将海绵体填充到好氧池和厌氧池中。该系统对BOD的去除率几乎超过90%。分别对好氧池和厌氧池的细菌植物群进行了分析。细菌植物群和BOD测定结果表明，拟杆菌门细菌在好氧池中对BOD的去除起主要作用，拟杆菌门和β-变形菌纲的细菌对厌氧池中BOD的去除起主要作用，本文采用荧光原位杂交和定量PCR的方法分析细菌数量的变化对BOD的影响本研究建议在今后的研究中予以删除。

The laboratory-scale model zero emission oriented toilet system was operated with the human excrement until 2005. In 2006, the toilet system was operated with the artificial sewage which consisted of peptone, yeast extract, cellulose and NaCl instead of the human excrement, because the components of the human excrement daily changed. And the wood chips or the sponge cubes were packed in a treatment tank as bacterial supports. The results were that the removal of BOD in the wood chips and sponge cubes was 83 and 67%, respectively. The wood chips were necessary to replenish, since the chips were digested by bacteria. The changing the direction of the flow tract was observed in the sponge cubes tank, so it could be considered the reason of less BOD removal.In 2006, the treatment tank was improved to two levels of aerobic tank and anaerobic tank that filled up sponge cubes. The BOD removal rates in this system almost exceeded 90%. The bacterial flora of the aerobic and anaerobic tank was analyzed, respectively. The results of the bacterial flora and the measurement of the BOD were that the bacteria in the phylum Bacteroidetes carried a main role of the BOD removal in the aerobic tank, and that the bacteria in the phylum Bacterioidetes and the class Betaproteobacteria carried a main role of the BOD removal in the anaerobic tank.I am going to perform FISH method and quantitive PCR to analyze a change of bacterial quantity to affect the BOD removal suggested by this study in future.