Analyses of the influence of maternal stress on the implantation of mouse embryo

背景：卵巢内分泌因子对着床成功起重要作用。然而，有些患者即使卵巢功能正常，足以诱导子宫内膜分化为妊娠、蜕膜化做准备，但仍未能妊娠。这些患者，种植失败，是难以治疗的，尽管最近的发展，艺术。众所周知，慢性应激诱导各种生殖障碍，包括排卵和流产。然而，母体应激对胚胎着床的影响尚不清楚。结果：本研究首先检测了应激对正常妊娠小鼠、假孕小鼠和胚胎移植小鼠生殖功能的影响。两组孕妇的压力均导致妊娠丢失。然而，这些发现是应激诱导的卵巢功能障碍的结果，而不是植入的结果。 ...更多信息 自己的病。我们接下来研究了应激对延迟着床模型的影响。该模型在卵巢切除后外源性地替代卵巢功能，因此似乎有可能在不影响卵巢功能的情况下研究应激对胚胎着床的影响。然而，卵巢切除术的手术应激似乎掩盖了随后的慢性应激的影响。因此，我们准备了卵巢切除雌性小鼠，并在补充外源性卵巢类固醇后移植小鼠囊胚。通过在围着床期给予雌二醇/孕酮的同时给予雌二醇预处理，与传统的假孕和胚胎移植模型相比，我们成功地诱导了令人满意的着床率。与对照组相比，胚胎移植前3d开始的持续超声应激显著抑制了植入率。目前，我们正在利用基因芯片技术研究这种应激诱导小鼠着床失败的基因表达谱。结论：我们可以证明应激本身诱导小鼠着床失败。进一步的研究可能会阐明这种应力诱导的种植失败的机制，这将导致一种新的治疗策略的发展原因不明的种植失败。少

Background : It has been proposed that ovarian endocrine factors play an important role for the successful implantation. However, some patients fail to pregnant even though their ovarian function appears normal and enough to induce endometrial differentiation to prepare pregnancy, decidualization. Such patients, implantation failure, are difficult to treat in spite of the recent development of ART. It is well known that chronic stress induces various reproductive disorder including aovulation and abortion. However, the influence of maternal stress on embryo implantation is not well clarified. Therefore, we analyzed whether maternal stress affect the mouse embryo implantation.Results : At first, we examined the influence of stress on the reproductive function of normal pregnant or pseudopregnant and embryo-transferred mouse. Maternal stress induced pregnancy loss in both groups. However, these findings were the results of stress induced ovarian dysfunction rather than the implantation f … More ailure itself. We nextly examined the influence of stress on delayed implantation model. This model replaces ovarian function exogenously after ovariectomy, so it appeared possible to investigate the influence of stress on embryo implantation without affecting ovarian function. However, surgical stress of ovariectomy appeared to obscure the influence of subsequent chronic stress.Therefore, we prepared ovariectomized female mice and transferred murine blastocysts after supplementation of exogenous ovarian steroids. By the pretreatment with estradiol in addition to estradiol/ progesterone administration during peri-implantation period, we succeeded to induce satisfactory implantation rate compared with conventional pseudopregnant and embryo transfer model Using this mouse model, we examined the influence of maternal stress on the implantation of mouse embryo. Continuous ultrasonic stress from 3days before the embryo transfer significantly suppressed implantation rate compared with control group. Presently, we are investigating the gene profile of this stress induced mouse implantation failure using cDNA microarray analysis.Conclution : We could demonstrated that stress itself induces implantation failure in mouse. Further investigation might clarify the mechanism of this stress induced implantation failure, which would lead to the development of a novel treatment strategy of unexplained implantation failure. Less