Transcriptional control of bacterial sporulation

细菌孢子形成是导致形成高抗性孢子的发育程序。该程序的执行取决于由RNA聚合酶（RNAP）和替代σ因子级联介导的基因表达的协同变化。在这里，我们提出了初步的结果，揭示了惊人的影响RNAP，δ和ω的非必需亚基，在枯草芽孢杆菌的孢子形成的效率。因此，我们建议系统地探讨枯草芽孢杆菌中RNAP的结构和功能及其组成/相互作用组在芽孢形成过程中的动态，重点是RNAP，δ，ω，σ因子和其他相关蛋白之间的相互作用。该项目将为我们理解孢子形成过程中转录机制的机制方面奠定基础，孢子形成过程广泛用于食品，饲料和制药应用，以及病原体的性质，如炭疽芽孢杆菌。

Bacterial sporulation is a developmental program that results in formation of highly resistant spores. The execution of this program depends on concerted changes in gene expression that are mediated by RNA polymerase (RNAP) and a cascade of alternative σ factors. Here we present preliminary results revealing surprising effects of non-essential subunits of RNAP, δ and ω, on the efficiency of sporulation in Bacillus subtilis. Consequently, we propose to systematically explore the structure and functioning of RNAP and the dynamics of its composition/interactome during sporulation in Bacillus subtilis, focusing on the interplay between RNAP, δ, ω, σ factors and other associating proteins. This project will establish a basis for our understanding of the mechanistic aspects of the transcriptional machinery during sporulation, a process extensively utilized in food, feed and pharmaceutical applications, and a property of pathogens, such as Bacillus anthracis.