Flowcytometer for high dimensional analytics (64 channels) and multiwell uptake

流式细胞术技术已经成为Klinikum rechts der Isar的比德斯坦校区的“主要工作人员”。除了血液分析，以前单细胞悬浮液中提取的组织细胞也越来越多地被用于分析。免疫学研究的限制因素仍然是人类细胞材料的有限获取，因此可用颜色的数量对比德斯坦校区的所有研究人员来说都是极其重要的。因此，现在可能扩展到64种颜色确实是一个根本性的进步。即将到来的一个重要挑战是研究从组织中分离出来的细胞，将其置于单细胞悬浮液中，并用流式细胞术使用表面标记物进行表征。此外，基于分泌产物的功能参数允许功能结论。组织细胞和组织的先天免疫细胞（呼吸道、痰、皮肤活检、肠道活检）是多种多样的（树突状细胞、先天淋巴细胞、γ δ t细胞）。气道和皮肤屏障的基础干细胞分析对细胞数量较少的分子分化要求较高。此外，在上皮细胞中有很强的自身荧光，现在可以很容易地管理，甚至用于细胞鉴定。现有的设备也是当时通过DFG大规模设备应用获得的-该设备对校园的必要性是毋庸置疑的。不幸的是，旧设备（经过近10年的使用）不再可靠地工作，造成巨大的维护成本，激光器也在他们的寿命结束。然而，所要求的设备（除了降低运营成本和替换旧设备外）也是一个重要的创新元素，因为它消除了技术限制，开辟了新的研究可能性，最终将促进有限细胞材料的研究，从而为比德斯坦校区的临床研究提供新的动力。

Flow cytometric technology has become a "main workhorse" on the Biederstein campus of the Klinikum rechts der Isar. In addition to blood analyzes, cells from tissue that have previously been brought into single-cell suspension are also increasingly being analyzed. The limiting factor in immunological research is and will remain the limited access to human cell material and therefore the number of available colors is extremely important for all researchers at the Biederstein campus. The now possible expansion to up to 64 colors is therefore really a fundamental step forward. An important upcoming challenge is the research of cells that are isolated from the tissue, brought into single cell suspension and characterized with flow cytometry using surface markers. Furthermore functional parameter based on secreted products allow functional conclusions. Tissue cells and innate immune cells of the tissue (brushings of the respiratory tract, sputum, skin biopsies, intestinal biopsies) are diverse (dendritic cells, innate lymphocytes, gamma delta-T cells). Basal stem cell analysis of airway and skin barrier place high demands on molecular differentiation with a small number of cells. In addition, there is strong autofluorescence in epithelial cells that can now be easily managed and even utilised for cell identification.The existing device was also acquired at the time through a DFG large-scale device application - the necessity of this equipment for the campus was beyond question. Unfortunately, the old device (after almost 10 years of use) no longer works reliably, causes immense maintenance costs and the lasers are also at the end of their life. However, the requested device is (in addition to the lower operating costs and replacement of the old one) also an important innovation element, as it removes technical limitations, opens up new research possibilities and will ultimately facilitate research with limited cell material and thus new impulses for clinical research at the Biederstein campus will put.