Accelerating the genetic improvement of grain legumes for Australia by developing doubled haploid technology for field pea and chickpea.

双单倍体技术被用于许多大田作物品种，以加速品种发育和创造纯合子群体进行遗传定位。田豌豆和鹰嘴豆对这种技术没有反应，但西澳大学的研究人员最近取得了突破，从体外培养的未成熟花粉中获得了单倍体前胚胎。进一步胚胎成熟的障碍已经确定，我们建议克服使用强大的显微镜工具来阐明单倍体胚胎学过程。这些信息将用于开发世界上第一个这些物种的体外双倍单倍体方案，这将促进更好的适应性，高产品种的开发和加速向工业交付。

Doubled haploid technology is used in many broad acre crop species to accelerate cultivar development and create homozygous populations for genetic mapping. Field pea and chickpea have been unresponsive to this technique but a recent breakthrough by UWA researchers has resulted in haploid pro-embryos from in vitro cultured immature pollen. A barrier to further embryo maturation has been identified, which we propose to overcome using powerful microscopy tools to elucidate haploid embryology processes. This information will be applied to develop world-first in vitro doubled haploid protocols for these species, which will facilitate the development and accelerated delivery to industry of better adapted, high yielding cultivars.