UHPLC coupled with a single quadrupole mass spectrometer for high throughput screening

所要求的是与质谱仪耦合的超高效液相色谱，用于有机分子的高通量层析筛选，在某些情况下，还包括小肽。用于快速层析分离的超高效液相系统在这里是至关重要的。特别是，超高效液相系统应配备多采样器，可以非常快速地(10秒周期)从微量滴定板上取样，并能够在非常高的压力(1000巴)下进行分析。质谱仪应配备电喷雾电离作为离子源，单四极杆作为分析器。为了以高通量、高质量选择性的色谱方式测定低浓度的分析物，对LC/MS系统进行了优化，使其循环时间短、灵敏度高、样品数量多、压力高。如果这一提议得到积极响应，LC/MS系统将使比勒费尔德大学的各种研究项目成为可能。科学上，我们的目标是开发i)有机合成的新方法，ii)新的非天然酶功能，以及新的酶工程方法。

Requested is a uHPLC coupled to a mass spectrometer for high-throughput chromatographic screening of organic molecules and, in some cases, small peptides. The uHPLC system for rapid chromatographic separation is of key importance here. In particular, the uHPLC system shall be equipped with a multisampler that can apply samples very rapidly (<10s cycle time) from microtiter plates and enables analysis at very high pressure (>1000 bar). The mass spectrometer shall be equipped with electrospray ionization as ion source and single quadrupole as analyzer. In order to determine low concentrations of analytes in a high throughput, mass-selective, chromatographic manner, the LC/MS system is optimized for short cycle times, high sensitivity, large number of samples and high pressure. Given a positive response for this proposal, the LC/MS system will enable a variety of research projects at Bielefeld University. Scientifically, we aim to develop i) new methods in organic synthesis, ii) new non-natural enzyme functions, as well as new enzyme engineering methods.