Superresolution SIM/SMLM Fluorescence Microscope

超分辨率荧光显微镜（SRFM）已经在许多领域彻底改变了对生物学的理解，因为它可以用近分子，但至少是纳米级的分辨率来成像自然环境中的动态过程。这些分辨率能力以前是为电子显微镜（EM）或原子力显微镜（AFM）保留的。这些开发得到了商业srfm开发的大量支持，这些srfm以日益复杂的软件和用户友好性为特征。在软物质研究、超分子体系和聚合物研究领域，电子显微镜和原子力显微镜迄今为止主导了结构分析，但大多数技术只能研究干燥、表面固定化或固定的样品（低温透射电镜或液细胞原子力显微镜有轻微例外）。共聚焦显微镜平行使用，但它的分辨能力遵循衍射极限。然而，在软物质中，以最精细的纳米分辨率对动态过程进行成像以及同时对动态变化下的水合状态进行成像也变得越来越重要。近年来，在软物质研究中，特别是在超分子系统、聚合物自组装和DNA纳米科学领域，SRFM也取得了开创性的成果，这些成果清楚地表明，使用SRFM方法可以实现一个全新的、更深层次的理解。因此，本GG提案的目标是购买一个结合结构化照明（SIM）和单分子定位显微镜（SMLM）方法的SRFM仪器，用于研究软物质系统。这项应用由A. Walther教授领导，但该仪器将隶属于美因茨电子显微镜中心，并将向一般用户开放。在这种情况下，总检察长瓦尔特还将填补一个学术职位（永久职位），这将确保日常的科学和行政运作。根据研究理念，其主要应用领域是DNA纳米科学、聚合物研究、胶体化学、肽纳米结构和生物化学等领域的自组装和纳米结构系统。仪器将加强外围设备测量温度引起的转变和自动流体路由研究软物质系统。州政府方面的资金将来自于为Walther教授提供的古腾堡研究教授职位，这是他被任命为JGU美因茨的一部分。

Super-resolution fluorescence microscopy (SRFM) has revolutionized the understanding in biology in many places, as it can be used to image dynamic processes in native environments with near molecular, but at least nanoscopic resolution. These resolution capabilities were previously reserved for electron microscopy (EM) or atomic force microscopy (AFM). These developments are massively supported by the development of commercial SRFMs, which feature increasingly sophisticated software and user-friendliness. In the areas of soft matter research, supramolecular systems, and polymer research, EM and AFM have dominated structural analysis to date, but most techniques can only study dried, surface-immobilized, or fixed samples (with slight exceptions for cryo-TEM or liquid-cell AFM). Confocal microscopy is used in parallel, but its resolving power follows the diffraction limit. However, imaging of dynamic processes with finest nanoscopic resolution and simultaneous imaging of hydrated states under dynamic changes is also of increasing importance in soft matter. There has also been seminal work on SRFM in soft matter research in recent years, particularly in the areas of supramolecular systems, polymer self-assembly, and DNA nanoscience, which clearly demonstrate that a completely new and deeper level of understanding can be achieved using SRFM methods. Therefore, the goal of this GG proposal is to purchase a SRFM instrument with combined Structured Illumination (SIM) and Single Molecule Localization Microscopy (SMLM) methodologies for the investigation of soft matter systems. This application is led by Prof. A. Walther, but the instrument will be affiliated with the Electron Microscopy Center Mainz and will be available to users in general. In this context, the AG Walther will also still fill an academic position (permanent position available), which will ensure the daily scientific and administrative operation. According to the research concepts, the main application fields are self-assembling and nanostructured systems from the fields of DNA nanoscience, polymer research, colloid chemistry, peptide nanostructures and biochemistry. The instrument will be strengthened with peripherals for measuring temperature-induced transitions and automated fluid routing for investigation of soft matter systems. Funding on the state side will come from a Gutenberg Research Professorship for Prof. Walther as part of his appointment to JGU Mainz.