Functional genomics using transgenic zebrafish

我们利用Tol 2转座子系统成功地开发了基因捕获、增强子捕获、Gal 4-UAS系统和体内转座系统等新的遗传学方法。利用这些方法，我们创造了600多个转基因鱼系，在特定的组织，细胞和器官中表达GFP或Gal 4。通过对这些转基因鱼的分析，我们研究了重要发育基因的功能。此外，我们还建立了转基因鱼类资源数据库，并促进了与世界各地研究人员的合作。因此，我们的研究成果极大地促进了脊椎动物基因研究的进展。

We successfully developed novel genetic methods by using the Tol2 transposon system such as gene trapping, enhancer trapping, the Gal4-UAS system and in vivo transposition system. By utilizing these methods, we created more than 600 of transgenic fish lines that express GFP or Gal4 in specific tissues, cells and organs. By analyzing these transgenic fish, we studied the function of important developmental genes. Further, we constructed a database of the transgenic fish resource and facilitated collaboration with researchers all over the world. Thus, our achievements greatly contributed advances of the study of vertebrate genes.