Triple quadrupole mass spectrometer with nano chromatography system

液相色谱法和质谱法的结合，通常被简称为LC-MS，可以对小分子和大分子进行高度特异性和敏感性的鉴定和定量。它现已成为诊断和细胞分子生物学研究中广泛使用的标准方法。靶向LC-MS分析旨在对选定的蛋白质或代谢物进行定向分析，可以在体液，组织和细胞中以高度特异性和极其敏感的方式进行识别和精确定量，直至低剂量水平。理想情况下，这些分析是用由微或纳米olc和三重四极杆质谱组成的LC-MS系统进行的，这些系统是为选择/多重反应监测（SRM， MRM）而设计的。这些LC-MS系统和相关的方法学越来越多地被用来代替抗体检测和定量蛋白质。一提到蛋白质和MRM技术，人们就会说“大规模西化”。这里提出的设备申请旨在为研究核心设施（RCF）蛋白质组学申请一种新型的三重四极柱型纳米olc LC-MS，它可以在最高水平上进行靶向质谱分析，用于蛋白质的检测和定量。RCF-Proteomics自2004年以来一直存在，并为MHH的许多工作组和自己的科学项目进行基于质谱的蛋白质和蛋白质组学分析。在100多个项目中，每年使用目标和非目标方法分析数千个样本。目前，只有近10年的LC-MS系统可用于靶向分析，该系统在16年前进入市场。为了让RCF-Proteomics继续支持和推进MHH的优秀研究，有针对性的LC-MS分析，并在未来实现新的，非常有趣的项目，一个新的，最重要的是更敏感的纳米olc三重四极杆MS是必要的。要获得的系统应该具有离子迁移模块和Q3中的陷阱功能，可以对整个质量范围进行扫描和MS3测量。在不明确的情况下，这对于验证测量数据和增加特异性非常重要，特别是对于蛋白质的定向分析，因此是必不可少的选择。

The combination of liquid chromatography and mass spectrometry, commonly referred to by the acronym LC-MS, enables the highly specific and sensitive identification and quantification of small and large molecules. It has now become a widely used standard method both in diagnostics and in cell and molecular biology research. Targeted LC-MS analyzes aim at the directed analysis of selected proteins or metabolites, which can be identified and precisely quantified in a highly specific and extremely sensitive manner in body fluids, tissues and cells down to the low atomol level. Ideally, these analyzes are carried out with LC-MS systems consisting of micro- or nanoLC and triple quadrupole MS, which are designed for selected / multiple reaction monitoring (SRM, MRM). These LC-MS systems and the associated methodology are increasingly being used instead of antibodies for the detection and quantification of proteins. In connection with proteins and MRM technology, one speaks of “mass western”.The equipment application presented here is intended to apply for a new LC-MS of the triple quadrupole type with nanoLC for the Research Core Facility (RCF) proteomics, which enables targeted MS analyzes at the highest level for the detection and quantification of proteins. RCF-Proteomics has existed since 2004 and carries out MS-based analyzes of proteins and proteomes for many working groups at the MHH and in own scientific projects. In more than 100 projects, several thousand samples are analyzed annually with targeted and untargeted methods.Currently, only an almost 10-year-old LC-MS system is available for taregted analysis, which came onto the market over 16 years ago. In order for RCF-Proteomics to continue to support and advance the excellent research at the MHH with targeted LC-MS analyzes and to enable new, highly interesting projects in the future, a new and above all more sensitive nanoLC triple quadrupole MS is necessary. The System to procure should have an ion mobility module and a trap function in Q3, which enables scans over the entire mass range and MS3 measurements. In unclear cases, this is important to validate the measurement data and to increase the specificity, especially for the directed analysis of proteins, and is therefore an essential option.