A systems biotechnology approach for improving substrate uptake during succinic acid production in a two-phase cultivation process

虽然利用细菌生物技术生产琥珀酸(丁二酸酯)已经得到了很好的研究，但在生物反应器中很难实现高产量。一个尚未被研究的方面是细菌细胞对葡萄糖等底物的摄取。在初步调查中，我们已经用改进的摄取系统检查了一些菌株变种，但数据无法系统收集。结合开发厌氧生长和生产行为的动态数学模型的理论方法，计划中的项目的重点是改善底物吸收和提高琥珀酸的生产。工作计划的要点包括新菌株变种的开发和实验测试，将测量数据整合到一系列模型中，以及设计和优化生物反应器中生产琥珀酸的两阶段流程。

Although the biotechnological production of succinic acid (succinate) with bacteria has already been very well-investigated, high productivity has hardly been achieved in the bioreactor. An aspect that has not yet been investigated, is the uptake of substrates such as glucose in the bacterial cells. In preliminary investigations, we have already examined some strain variants with modified uptake systems, but data could not be collected systematically. In conjunction with a theoretical approach in which a dynamic, mathematical model for the anaerobic growth and production behaviour is to be developed, the focus of the planned project is on improved substrate uptake and improved production of succinic acid. Essential points of the work program consist of the development and experimental testing of new strain variants, the integration of measured data into an ensemble of models and the design and optimization of a two-stage process for the production of succinic acid in the bioreactor.