laser microdissection microscope

所要求的显微镜和附件旨在用于CECAD的蛋白质组学、转录组学和成像核心设施。下一代测序(NGS)和质谱学的最新发展使得对来自少量组织、单细胞甚至是孤立核的样本进行无偏分析成为可能。为了以自动化、高通量的方式分离特定的细胞群体、单个细胞及其亚室，现代激光显微解剖(LMD)系统已与深度学习和基于机器学习的成像分析工具相结合。因此，购置一台现代LMD显微镜将是科隆校区研究小组开展的项目的一项重要资产。此外，由于蛋白质组学和转录组学所需的样本数量很大，因此将自动化和高效的细胞分类系统与高分辨率成像相结合是很重要的。NGS和灵敏质谱仪的平台在科隆建立得很好，可以分析复杂的生物样品。由于对单个细胞群体的定量分析的需求大幅增加，新的LMD显微镜对于实现计划中的研究项目将是必不可少的。

The microscope and accessories requested are intended for use in the Proteomics, Transcriptomics, and Imaging Core Facilities at CECAD. Recent developments in next-generation sequencing (NGS) and mass spectrometry have enabled the unbiased analysis of samples from small amounts of tissue, single cells, and even isolated nuclei. To isolate specific cell populations, individual cells and their subcompartments in an automated, high-throughput manner, modern laser microdissection (LMD) systems have been combined with deep-learning and machine learning-based imaging analysis tools. The acquisition of a modern LMD microscope will therefore be a vital asset to the projects carried out by the research groups at the Cologne campus. Furthermore, due to the high number of samples required for proteomics and transcriptomics, it is important to integrate an automated and efficient cell classification system with high-resolution imaging. Platforms for NGS and sensitive mass spectrometers are well-established in Cologne and enable analysis of complex biological samples. Since the demand for quantitative analyses of individual cell populations has massively increased, a new LMD microscope will be essential to the realization of the planned research projects.